Research progress on utilizing single cell sequencing technology to investigate immune cell changes in IgA nephropathy
IgA nephropathy(IgAN)is the most common form of glomerulonephritis globally.Nearly all patients develop end-stage renal failure within their lifetime.Abnormal immune system,increased glycosylation deletion of IgA1,and the deposition of IgA in the mesangial region of the kidneys play an important role in the pathology of IgAN.Although traditional high-throughput sequencing technology has provided vital evidence and insights into the mechanism of IgAN,it has limitations in revealing cellular heterogeneity.With advancements in molecular biology,microfluidics,and bioinformatics,single-cell sequencing technologies have been developed and applied.The technologies can explore cellular states under physiological and disease abnormalities at the single-cell level,offer high-resolution and rich data essential for disease characterization and understand complex interactions between individual cells.In recent years,a number of important achievements in the application of single-cell sequencing technology have been published.These a-chievements include the analysis of differences in peripheral immune cell expression and subset changes,the mapping of the renal tran-scriptome,and the revelation of the communication network between IgAN cells.We also have applied the technologies to identify B cell subsets and their pathway alterations associated with IgAN disease progression.This article reviews the application of single-cell analysis technology in IgAN research.It also reviews the progress and inspiration in the analysis of the pathogenesis and progression mechanisms as well as potential therapeutic targets of IgAN.
IgA nephrologySingle-cell sequencingImmunityIntercellular communication
NETL
NSTL
万方数据
