Observation of application of TGF-β1 combined with ADA and GeneXpert MTBRIF in the diagnosis of tuberculous pleural effusion
Objective To observe the application effects of transforming growth factor-β1(TGF-β1)combined with adenosine deaminase(ADA)and GeneXpert MTB/RIF in the diagnosis of tuberculous pleural effusion(TPE).Methods Two hundred and twenty patients who came to the hospital due to exudative pleural effusion from August 2020 to August 2022 were selected as research subjects.The final pathological examination confirmed that there were 92 patients with TPE(TPE group),68 with bacterial pleural effusion(BPE)(BPE group)and 60 with malignant pleural effusion(MPE)(MPE group).The TGF-β1 and ADA in pleural effusion were detected.GeneXpert MTB/RIF in pleural effusion was conducted.The diagnostic value of pleural effusion TGF-β1 and ADA alone and in combination on TPE was analyzed and the best cut-off values were determined.The consistency of TGF-β1,ADA,GeneXpert MTB/RIF alone and in combination with pathological diagnosis was analyzed.Results The levels of TGF-β1 and ADA in pleural effusion were the highest in the TPE group,followed by the BPE group,and the lowest in the MPE group(P<0.05).ROC curve analyses showed that the best cut-off values of TGF-β1 and ADA in the diagnosis of TPE were 31.155ng/L and 28.495U/L,re-spectively.The corresponding areas under the curves(AUCs)were0.830 and0.851,respectively,and the combined diagnosis was 0.904.The consistency Kappa value of the combined detection of TGF-β1 and ADA with pathological diagnosis was 0.76.The consistency Kappa value of GeneXpert MTB/RIF with pathological diagnosis was 0.71.The consistency Kappa value of the combined diagnosis of TGF-β1,ADA and GeneXpert MTB/RIF with pathological diagnosis was 0.83.Conclusions The levels of TGF-β1 and ADA in pleural effusion of patients with TPE are higher than those of patients with bacterial and malignant types of pleural effusion.GeneXpert MTB/RIF combined with pleural effusion TGF-β1 and ADA detection has a high diagnostic value on TPE.It also has a good consistency with pathological diagnosis.
Transforming growth factor-β1Adenosine deaminaseGeneXpert MTB/RIFTuberculous pleural effusionDiagnostic value
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