Analysis of pulp healing and influencing factors after autologous tooth transplantation
Objective To explore the dental pulp healing after tooth autotransplantation and its influencing factors.Methods Nine Beagle dogs'incisors were used as experimental teeth and randomly divided into group A,B and C,3 teeth in each group.A root canal file was used to enlarge the apical hole to a diameter＞1mm in the group A.A 3mm apical amputation and a root canal filling were used in the group B.The teeth were not treated in the group C.After tooth extraction,autologous transplantation was performed.The clinical manifestations of transplanted teeth were observed after 1 week,1 month and 6 months of operation.The changes and healing of pulp tissue were observed by X-ray,Micro-CT and histopathological staining.Results One tooth fell out in the group A and C,two teeth fell out in group B,and the rest teeth were loose by 0～Ⅱ°.No swelling of pus was found in the gums.In the group A,had enlarged apical foramen,reduced low-density shadows around the apex,spotted high-density shadows visible on the apical apex and lateral walls of the root canal,and vascularized active connective tissue in the medullary cavity visible in HE staining.High density shadows could be seen at the root apex of the group B,with external absorption of the tooth root.Blocky high-density shadows could be seen at the top of the pulp chamber,the lateral wall of the root canal,and the root apex.Histologically,new connective tissue and osteoid like substances could be seen in the pulp cavity deposited on the root canal wall.Low density shadowscould be seen at the apex of the group C,and histologically,necrosis of pulp tissue could be observed in the pulp cavity.Inflammatory absorption areas can be seen in the root.Conclusions The size of the apical foramen of the donor teeth and the sealing of the apical foramen to isolate infection may promote the pulp healing and pulp regeneration of the transplanted teeth.
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