结直肠癌患者肠道菌群及粪便SDC2基因甲基化检测意义及其关系研究
The significance and relationship of detection of gut microbiota and fecal SDC2 gene methylation in colorectal cancer patients
钟莉华 1张德文 1朱喜丹 2刘霞 1胡家业1
作者信息
- 1. 四川省自贡市第三人民医院检验科,四川 自贡 643000
- 2. 西南医科大学附属医院检验科,四川 泸州 646000
- 折叠
摘要
目的 探讨粪便Syndecan-2(SDC2)甲基化与肠道菌群失调在结直肠癌(CRC)中的意义.方法 将70 例CRC患者(CRC组)、65 例结直肠腺瘤性息肉(CAP)患者(CAP组)及 50 例健康志愿者(对照组)作为研究对象,检测其粪便DNA SDC2 甲基化与肠道菌群失调情况,实时荧光定量PCR法定量分析粪便菌群,Spearman相关系数分析CRC患者粪便SDC2 甲基化与其定量分析结果之间的相关性.结果 实时荧光定量PCR法在分析粪便菌群中的稳定性及特异性良好,CRC组粪便SDC2 甲基化阳性率及肠道菌群失调率均高于对照组与CAP组(P＜0.05);与对照组相比,CRC组及CAP组有益菌定量分析水平降低,CRC组肠道菌群中有害菌水平上升(P＜0.05).CRC患者粪便SDC2 甲基化与肠道乳酸杆菌、双歧杆菌定量水平呈负相关(P＜0.05),与大肠杆菌、粪肠球菌以及脆弱拟杆菌定量分析水平呈正相关(P＜0.05).结论 CRC患者存在明显的肠道菌群紊乱表现及高水平SDC2 甲基化阳性率,肠道菌群可能通过影响SDC2 甲基化参与CRC发生与发展.
Abstract
Objective To investigate the significance of fecal Syndecan-2(SDC2)methylation and intestinal flora disorder in patients with colorectal cancer(CRC).Methods Seventy patients with CRC were selected as a CRC group.Sixty-five patients with colorectal adenomatous polyp(CAP)were selected as a CAP group.Another 50 healthy volunteers were selected as a control group.The fecal DNA SDC2 methylation and intestinal flora disorder were detected.Real-time fluorescence quantitative PCR was used to quantitatively analyze the fecal flora.Spearman correlation coefficient was used to analyze the correlation between fecal SDC2 methylation and quantitative analysis results of fecal flora in the CRC patients.Results Real-time fluorescence quantitative PCR method had good stability and specificity in quantitative analysis of fecal flora.The positive rate of fecal SDC2 methylation and intestinal flora disorder rate in the CRC group were higher than those in the control group and the CAP group(P＜0.05).Compared with the control group,the quantitative analysis levels of beneficial bacteria in the CRC group and the CAP group were decreased,and the levels of pernicious bacteria were risen in the CRC group(P＜0.05).The fecal SDC2 methylation was negatively correlated with quantitative levels of intestinal Lactobacillus and Bifidobacterium in the CRC group(P＜0.05).However,it was positively correlated with quantitative levels of Escherichia coli,Enterococcus faecalis and Bacteroides fragilis(P＜0.05).Conclusions CRC patients have obvious intestinal flora disorder and high positive rate of SDC2 methylation.Intestinal flora may participate in the occurrence and devel-opment of CRC by affecting SDC2 methylation.
关键词
结直肠癌/SDC2甲基化/肠道菌群失调Key words
Colorectal cancer/SDC2 methylation/Intestinal flora disorder引用本文复制引用
基金项目
自贡市科学技术局科研基金资助项目(2020ZC20)
出版年
2024
NETL
NSTL
万方数据