The significance and relationship of detection of gut microbiota and fecal SDC2 gene methylation in colorectal cancer patients
Objective To investigate the significance of fecal Syndecan-2(SDC2)methylation and intestinal flora disorder in patients with colorectal cancer(CRC).Methods Seventy patients with CRC were selected as a CRC group.Sixty-five patients with colorectal adenomatous polyp(CAP)were selected as a CAP group.Another 50 healthy volunteers were selected as a control group.The fecal DNA SDC2 methylation and intestinal flora disorder were detected.Real-time fluorescence quantitative PCR was used to quantitatively analyze the fecal flora.Spearman correlation coefficient was used to analyze the correlation between fecal SDC2 methylation and quantitative analysis results of fecal flora in the CRC patients.Results Real-time fluorescence quantitative PCR method had good stability and specificity in quantitative analysis of fecal flora.The positive rate of fecal SDC2 methylation and intestinal flora disorder rate in the CRC group were higher than those in the control group and the CAP group(P＜0.05).Compared with the control group,the quantitative analysis levels of beneficial bacteria in the CRC group and the CAP group were decreased,and the levels of pernicious bacteria were risen in the CRC group(P＜0.05).The fecal SDC2 methylation was negatively correlated with quantitative levels of intestinal Lactobacillus and Bifidobacterium in the CRC group(P＜0.05).However,it was positively correlated with quantitative levels of Escherichia coli,Enterococcus faecalis and Bacteroides fragilis(P＜0.05).Conclusions CRC patients have obvious intestinal flora disorder and high positive rate of SDC2 methylation.Intestinal flora may participate in the occurrence and devel-opment of CRC by affecting SDC2 methylation.
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