Analysis of correlation between serum LncRNA MEG3 level and diabetic nephropathy in patients with type 2 diabetes mellitus
Objective To explore the correlation between serum LncRNA MEG3 level and diabetic nephropathy(DN)in patients with type 2 diabetes mellitus(T2DM).Methods One hundred and forty T2DM patients were selected.They were divided into a normal albuminuria group(n=45;UACR＜30 mg/g),a microalbuminuria group(n=47;UACR30～300 mg/g)andalargeal-buminuria group(n=48;UACR＞300 mg/g)according to urinary microalbuminuria/creatinine ratio(UACR).Fifty healthy people who underwent physical examination in our hospital during the same period were selected as a control group.Clinical data of T2DM patients were collected.Serum LncRNA MEG3 levels were detected by real-time quantitative fluorescent PCR(qRT-PCR).The correlation between serum LncRNA MEG3 levels and clinical indicators as well as the diagnostic value of serum LncRNA MEG3 in DN were analyzed.Results Compared with the control group,the expression of serum LncRNA MEG3 in the normal albuminuria group,the microalbuminuria group and the macroalbuminuria group was significantly up-regulated(P＜0.05).Fasting blood glucose(FPG),fasting insulin(FIns),insulin resistance index(HOMA-IR),glycosylated hemoglobin(HbA1c),systolic blood pressure(SBP),blood creatinine(SCr),urinary UACR,transforming growth factor-β1(TGF-β1),Interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and serum LncRNA MEG3 in the microalbuminuria group and the macroalbuminuria group were higher than those in the normal albuminuria group,and those in the macroalbuminuria group were higher than those in the microalbuminuria group(P＜0.05).The diastolic blood pressure(DBP),total cholesterol(TC)and low density lipoprotein cholesterol(LDL-C)in the mac-roalbuminuria group were higher than those in the normal albuminuria group(P＜0.05).Serum LncRNA MEG3 levels were positively correlated with the course of T2DM,FPG,FIns,HOMA-IR,HbA1c,SBP,DBP,TC,LDL-C,SCr,UACR,IL-6,TNF-α and TGF-β1(P＜0.05).The AUC of serum LncRNA MEG3 level for the diagnosis of DN was 0.960(95%CI=0.913～0.986),and the diagnostic sensitivity and specificity were94.74%and84.44%,respectively with a threshold of 1.5.Conclusions Serum LncRNA MEG3 levels are significantly increased in patients with DN.It is correlated with glucose and lipid metabolism,insulin resistance and inflammatory indicators.It has a good diagnostic value for DN.
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