Analysis of the clinical significance of expressions of cell division cycle 20 gene in circulating tumor cells in patients with esophageal squamous cell carcinoma
Objective To investigate the clinical significance of expressions of cell division cycle 20(CDC20)gene in circulating tumor cells(CTCs)of patients with esophageal squamous cell carcinoma(ESCC).Methods From April,2020 to October,2022,72 patients with ESCC diagnosed by pathology and treated in our hospital were selected.Peripheral blood samples were collected before any treatment,and CTCs in the samples were separated and counted.The expressions of CDC20 mRNA in CTCs were analyzed by RT-qPCR.The patient's survival was recorded.The deadline for follow-up was August 2023.Results The median level(Q1,Q3)of CTCs 20 mRNA in the 72 patients with ESCC was 2.76(1.41,6.39).The patients were divided into a low expression group and a high expression group according to the expressions of CDC20.Compared with the low expression group,the tumor infiltration depth was T3/4 and the number of TNM stage Ⅲ/Ⅳ cases and CTCs count in the high expression group were higher(P＜0.05).Correlation analysis showed that there was a significant positive correlation between CTCs counts and CDC20 expressions(P＜0.001).During the follow-up period,9 patients died in the low expression group and 18 patients died in the high expression group.Kaplan-Meier curve showed that the survival rate of the high expression group was significantly lower than that of the low expression group(P＜0.05).Multivariate Cox model analysis showed that age,CTCs count,lymph node metastasis,and CDC20 level were in-dependent predictors of overall survival(P＜0.05).Conclusions The high expression of CDC20 in CTCs of ESCC patients is significantly correlated with tumor invasion depth,lymphatic metastasis,CTCs count,and poor survival outcome.Therefore,CDC20 in CTCs may be a useful index for the prognosis of ESCC patients.
万方数据
维普
