The Effect of Qingre Lishi Jianpi Formula(清热利湿健脾方)on Gut Microbiota,Immune Inflammatory Response,and Endoplasmic Reticulum Stress in Rats with Ulcerative Colitis
Objective To explore the effects of Qingre Lishi Jianpi Formula(清热利湿健脾方)on gut microbiota,immune in-flammatory response,and endoplasmic reticulum stress in rats with ulcerative colitis(UC).Method Forty male SD rats of SPF grade divided four groups,each group 10 rats.Prepare UC rat model.Low dose group rats were given 1.42 g/kg Qingre Lishi Jianpi Decoction by gavage for 14 days;High dose group rats were given 5.68 g/kg of Qingre Lishi Jianpi Formula by gavage for 14 days;the model group and normal group rats were gavaged with physiological saline at the same dosage for 14 days.Compare the DAI scores,gut microbiota,immune inflammatory factors,and expression of ATF4,CHOP,and GRP78 proteins in each group of rats.Result Compared normal group,the DAI score,relative expression levels of Escherichia coli and Enterococcus,IL-6,IL-17,ATF4,CHOP,and GRP78 protein expression gray values of UC rats in other groups increased,while Lactobacil-lus and Bifidobacterium relative expression,and IL-10 levels decreased(P＜0.05);Compared model group,the low-dose and high-dose groups showed a decrease in DAI scores,relative expression levels of Escherichia coli and Enterococcus,and grayscale values of IL-6,IL-17,ATF4,CHOP,and GRP78 protein expression in UC rats,while Lactobacillus and Bifidobac-terium relative expression,and IL-10 levels increased(P＜0.05);compared to the low-dose group,the high-dose group showed a decrease in the relative expression levels and IL-10 levels of Lactobacillus and Bifidobacterium in UC rats,while the relative expression levels and IL-10 levels of Lactobacillus and Bifidobacterium increased(P＜0.05).Conclusion The Qingre Lishi Jianpi formula can improve the intestinal microbiota of UC rats,alleviate immune inflammatory response,and downregulate the expression of ATF4,CHOP,and GRP78 proteins.
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