Study on the Effect of Qinggan Jiangzhi Formula on Intestinal Barrier in Rats with Metabolic Related Fatty Liver Disease Based on the Intestinal Liver Axis
Objective To investigate the effect of Qinggan Jiangzhi prescription on intestinal mucosal barrier in rats with metab-olism-related fatty liver disease(MAFLD)induced by high fat diet based on gut-liver axis theory.Method 60 SPF male SD rats,10 of which were randomLy selected as the normal group and the rest as the high-fat diet group,were fed with high-fat diet for 8 weeks.After successful modeling,The rats in the high-fat diet group were randomLy divided into model group,low dose group,medium dose group,high dose group and Shuilinjia group.The histopathological changes of liver and terminal ileum,serum biochemistry and endotoxin levels,and the expression of tight junction protein ZO-1 and Occludin in ileum tissues of rats in each group were observed.Result Compared with the normal group,the liver and ileum of rats in the model group showed obvi-ous pathological damage,The levels of ALT,TC,TG,LDL-C and LPS in serum were significantly increased(P＜0.05),and the positive expressions of ZO-1 and Occludin in ileum were decreased(P＜0.05).Compared with the model group,the levels of ALT,TC,TG and the content of LDL-C in the Shuilinjia group and the high and middle dosage of traditional Chinese medi-cine were significantly reduced(P＜0.05).The serum LPS level of rats in the Shuilinjia group and the traditional Chinese medi-cine group was significantly lower than that in the model group(P＜0.05).The positive expression of ZO-1 and Occludin pro-tein in the Shuilinjia group and the high and middle dosage groups of traditional Chinese medicine increased,while the protein expression in the low dosage group of traditional Chinese medicine increased slightly.Conclusion Qinggan Jiangzhi Recipe can regulate lipid metabolism in rats,repair intestinal tight junction,maintain normal intestinal mucosal barrier function,prevent in-testinal inflammatory factors,endotoxin,etc.from entering the liver,suggesting that the mechanism of Qinggan Jiangzhi Recipe in treating MAFLD may be related to regulating the intestinal hepatic axis and improving intestinal immune barrier.
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