Effect of Dendrobium Dendrobium Polysaccharide on the Inhibition of Liver Endoplasmic Reticulum Stress in Mice with Nonalcoholic Fatty Liver by Regulating Jak2/Stat5 Pathway
Objective To investigate the inhibitory effect of Dendrobium officinale polysaccharide on the stress of liver endo-plasmic reticulum in mice with nonalcoholic fatty liver by regulating JAK2/STAT5 pathway.Methods Fifty mice were randomLy divided into control group,model group,low-dose group(20 μg/mL),medium-dose group(40 μg/mL)and high-dose group(80µg/mL),with 10 mice in each group.Except for the control group,the other mice were induced with fatty liver model by high-fat diet,and the mice in the low-dose,medium-dose and high-dose groups were given different doses of Dendrobium offici-nale by gavage.After 8 weeks of continuous administration,liver tissue samples of mice in each group were taken for HE staining to detect serum liver function and blood lipid indexes of mice in each group,and the gene expressions of JAK2,STAT5,GRP78,CHOP,Caspase 12,Bcl-2 and Bax in liver tissue of mice were detected by RT-qPCR.Result The body weight,liver weight and liver index of mice in the model group and the various dose groups were significantly increased compared with the control group(P＜0.05),while the body weight,liver weight and liver index of mice in the treatment group were significantly decreased com-pared with the model group(P＜0.05),and showed a dose-dependent relationship.The AST,ALT,TC,TG,LDL-C of mice in the model group and the various dose groups were significantly increased compared with the control group(P＜0.05),while the HDL-C of mice in the various dose groups were significantly decreased compared with the model group(P＜0.05),and the HDL-C of mice in the various dose groups was significantly increased compared with the model group(P＜0.05),and showed a dose-dependent relationship.The HE staining results of liver tissue showed that the structure of hepatic lobules was seriously damaged in the model group,and a large number of fat vacuoles were gathered.The hepatic cells of mice in the D.candidum poly-saccharide groups showed obvious disordered arrangement and fat vacuoles aggregation,which were improved to varying degrees compared with the model group.The relative expression levels of JAK2,STAT5 and Bcl-2 genes in the liver tissue of mice in the model group and the various dose groups were significantly decreased(P＜0.05),while the relative expression levels of GRP78,CHOP,Caspase 12 and Bax genes were significantly increased(P＜0.05).The relative expression levels of JAK2,STAT5 and Bcl-2 genes in the liver tissue of mice in the D.candidum polysaccharide drug intervention groups were significantly increased(P＜0.05),while the relative expression levels of GRP78,CHOP,Caspase 12 and Bax genes were significantly decreased(P＜0.05),and showed an obvious dose-dependent relationship.Conclusion Dendrobium dendrobium polysaccharide can effectively improve liver injury in mice with nonalcoholic fatty liver,and the treatment is significantly dose-dependent,which may be related to the activation of JAK2/STAT5 pathway to inhibit endoplasmic reticulum stress.
dendrobium officinale polysaccharideJAK2/STAT5 channelnonalcoholic fatty liverliver ER stress
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