Exploring the Effect of Gaoyou Bashaga Water Extract on Breast Hyperplasia Based on Network Pharmacology and Experimental Validation
Objective It is to verify and analyze the mechanism of Gaoyou-Bashaga water extract(DSWE)in the treatment of mammary gland hyperplasia through network pharmacology and experiments.Methods DSWE related targets were searched through TCMSP and Swiss Target Prediction databases.Breast hyperplasia related genes were collected from GeneCards,NCBI and OMIM databases.PPI network was constructed and performed for their common targets.The Wistar rat mammary hyperplasia mod-el was constructed to determine the expression levels of estradiol and progesterone in serum with DSWE intervention by Elisa.Re-sults By constructing a component-disease-pathway-target network,it shows that six active components in DSWE can inhibit breast hyperplasia through multiple pathways.DSWE in the high-dose group could significantly reduce estradiol expression and increase the expression of progesterone.Conclusion DSWE can inhibit mammary gland hyperplasia through multi-component-multi-target-multi-pathway,which will provide preliminary experimental data support for the clinical application of DSWE in the treatment of mammary gland hyperplasia,but the in-depth molecular mechanism still needs to be further explored.
Gaoyou-Bashaga water extractnetwork pharmacologyhyperplasia of mammary glandsestradiolprogesterone
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