Exploring Molecular-Target Mechanism of Atherosclerosis Treatment with Qishen Yiqi Dropping Pills(芪参益气滴丸)Based on Network Pharmacology and Molecular Docking
Objective To investigate the molecular-targeting mechanism of Qishen Yiqi Dropping Pills(芪参益气滴丸)for the treatment of atherosclerosis using a network pharmacological analysis.Methods A reference high performance liquid chroma-tography(HPLC)study was conducted to obtain the components of Qishen Yiqi Dropping Pills,the active ingredients were screened using TCMSP and combined with literature,the active ingredient targets were obtained from TCMSP,SwissTarget Predic-tion database and SEA database.The atherosclerosis targets were obtained from GEO database.Cytoscape mapped the active in-gredient-intersection target.The active ingredient-interaction network was constructed by Cytoscape.The protein interaction network was constructed by STRING platform.GO/KEGG enrichment analysis was performed by Metascape platform,and molec-ular docking software Autodock was used to validate the active ingredients corresponding to the core targets by molecular docking.Results It screened 12 active ingredients of Qishen Yiqi Dropping Pills and their corresponding targets 478,370 atherosclerotic targets,7 core targets and 7 KEGG pathways were obtained,and molecular docking verified that the core targets and corresponding ingredients all had good binding activities.Conclusion Qishen Yiqi Dropping Pills can treat atherosclerosis by regulating signaling pathways such as chemokines,platelet activation and cell adhesion molecules,modulating immune response,and inhibiting inflam-mation and subendothelial migration of monocytes cells.
万方数据
维普
