Bioinformatics Research and Potential Chinese Medicine Prediction of Type 2 Diabetes Mellitus Complicated with Cerebral Infarction
Objective Based on bioinformatics methods,the key genes and potential effective traditional Chinese medicines of type 2 diabetes mellitus(T2DM)with cerebral infarction were explored.Methods T2DM gene chip data GSE29221 and cerebral infarction chip data GSE97537 were downloaded from GEO database to determine differential genes(DEGs).GO and KEGG a-nalysis of intersection DEGs were completed in Metascape database,and protein interaction network(PPI)analysis was comple-ted,and visual analysis was performed by Cytoscape-v3.8.Coremine Medical retrieval platform was used to screen out the po-tential Chinese medicines for the treatment of T2DM with cerebral infarction.Results According to the screening conditions,the GSE29221 chip contained 1445 DEGs(1156 up-regulated genes and 289 down-regulated genes).The GSE97537 chip con-tains 10842 DEGs(3884 up-regulated genes and 4080 down-regulated genes).Metascape online analysis showed that the bio-logical processes(BP)of DEGs were mainly involved in regulating smooth muscle cell proliferation,positively regulating protein phosphorylation and programmed cell death.Cell component(CC)mainly included extracellular matrix,vesicle cavity,etc.Mo-lecular function(MF)mainly affected cytokine activity,receptor ligand activity,growth factor activity,etc.At the same time,KEGG pathway enrichment analysis showed that T2DM complicated with cerebral infarction may be related to AGE-RAGE,PI3K-Akt,Foxo,Jak-STAT,MAPK and other signaling pathways or cascades,and may also involve H1F-1 signaling,fluid shear stress and atherosclerosis,endocrine resistance,apoptosis,longevity regulation pathway,insulin resistance and other biologi-cal processes.Coremine Medical analysis showed that Huangqi(Astragali Radix),Chuanxiong(Chuanxiong Rhizoma),Renshen(Ginseng Radix Et Rhizoma),Honghua(Carthami Flos),Danshen(Salviae Miltiorrhizae Radix Et Rhizoma),Chishao(Paeoniae Radix Rubra),Shengjiang(Zingiberis Rhizoma Recens),Taoren(Persicae Semen),Fuling(Poria)and Danggui(Angelicae Sinensis Radix)were closely related to key genes.Conclusion The pathogenesis of T2DM complicated with cerebral infarction may involve a complex mechanism of multiple pathways and multiple targets.Huangqi(Astragali Radix),Chuanxiong(Chuanx-iong Rhizoma)and Renshen(Ginseng Radix Et Rhizoma)are most closely related to the key genes of T2DM complicated with cerebral infarction.
type 2 diabetes complicated with cerebral infarctionkey genesChinese medicinebioinformatic analysis
万方数据
维普
