Determination of pKa Value of Pharmacodynamic Substance Basis Tectorigenin and Iristectorigenin A of Shegan(Belamcanda Chinensis)
Objective To determine the pKa values of tectorigenin and iristectorigenin A by high performance liquid chromatog-raphy and provide reference for the formulation design.Methods The mobile phase was acetonitrile phosphoric acid buffer solution with different pH values.At the detection wavelength of 265 nm,the retention factors of tectorigenin and iristectorigenin A were determined successively and then the pKa value was calculated.Results The pKa values of tectorigenin was 6.49 and that of iris-tectorigenin A was 6.51.Conclusion High performance liquid chromatography is a simple and effective method for the determina-tion of pKa value of tectorigenin and iristectorigenin A.
万方数据
维普
