姜黄素调控MAPK/NF-κB/AP-1信号通路抑制狼疮性肾炎小鼠炎性反应作用分析

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中文摘要：目的探讨姜黄素调控丝裂原活化蛋白激酶(MAPK)/核因子-κB(NF-κB)/激活蛋白-1(AP-1)信号通路抑制狼疮性肾炎(lupus nephritis,LN)小鼠炎性反应作用.方法 LN小鼠16只分为LN组和实验组,各8只;C57BL/6小鼠8只作为对照组.实验组小鼠给予200 mg/kg姜黄素灌胃,1次/d;LN组和对照组小鼠灌胃等体积氯化钠溶液,1次/d;各组小鼠连续灌胃4周.运用全自动生化分析仪测定Scr和BUN水平;运用BCA蛋白测定试剂盒说明书测定尿蛋白水平;运用ELISA法测定白细胞介素1β(IL-1β)、血清白细胞介素6(IL-6)和肿瘤坏死因子-α(TNF-α)水平;运用Western Blot法测定MAPK/NF-κB/AP-1蛋白表达.结果 LN组和实验组Scr和BUN水平高于对照组(P＜0.05);实验组Scr和BUN水平低于LN组(P＜0.05).LN组和实验组尿蛋白水平高于对照组(P＜0.05);实验组尿蛋白水平低于LN组(P＜0.05).LN组和实验组血清IL-1β、IL-6和TNF-α水平高于对照组(P＜0.05);实验组血清IL-1β、IL-6和TNF-α水平低于LN组(P＜0.05).LN组和实验组MAPK蛋白灰度值、NF-κB蛋白灰度值和AP-1蛋白灰度值高于对照组(P＜0.05);实验组MAPK蛋白灰度值、NF-κB蛋白灰度值和AP-1蛋白灰度值低于LN组(P＜0.05).结论姜黄素可通过下调MAPK/NF-κB/AP-1蛋白表达而抑制LN小鼠炎性反应,且可改善肾功能.

外文标题：Analysis of Curcumin Regulating MAPK/NF-κB/AP-1 Signaling Pathway and Inhibiting Inflammatory Response in Lupus Nephritis Mice

外文摘要：Objective To investigate the inhibitory effect of curcumin on the inflammatory response in lupus nephritis mice by regulating the mitogen activated protein kinase(MAPK)/nuclear factor kappa B(NF-κB)/activator protein-1(AP-1)sig-naling pathway.Methods 16 LN mice were divided into LN group and experimental group,each 8 mice;Eight C57BL/6 mice were used as the control group.The experimental group mice were given 200 mg/kg curcumin by gavage,once a day;LN group and control group mice were given equal volume of physiological saline by gavage,once a day;Each group of mice was orally ad-ministered continuously for 4 weeks.Using a fully automated biochemical analyzer to measure the levels of Scr and BUN;Use the instructions of the BCA protein assay kit to measure urinary protein levels;Using ELISA method to measure the levels of IL-1β,IL-6,and TNF-α;Using Western Blot method to determine the expression of MAPK/NF-κB/AP-1 protein.Results The LN group and experimental group levels of Scr and BUN higher control group(P＜0.05);experimental group levels of Scr and BUN lower LN group(P＜0.05).The LN group and experimental group urinary protein levels higher control group(P＜0.05);experimental group urine protein level lower LN group(P＜0.05).The LN group and experimental group serum levels of IL-1β,IL-6,and TNF-α higher control group(P＜0.05);experimental group serum levels of IL-1β,IL-6,and TNF-α lower LN group(P＜0.05).The LN group and experimental group gray values of MAPK protein,NF-κB pro-tein,and AP-1 protein higher control group(P＜0.05);experimental group gray values of MAPK protein,NF-κB protein,and AP-1 protein lower LN group(P＜0.05).Conclusion Curcumin can inhibit the inflammatory response of LN mice and im-prove renal function by downregulating the expression of MAPK/NF-κB/AP-1 protein.

外文关键词：

CurcuminMitogen activated protein kinaseNuclear factor-κBActivating protein-1Lupus nephritisin-flammatory reaction

作者：

屈红丽、刘曼丽、李响

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作者单位：

周口市中医院,河南周口 466000

关键词：

姜黄素丝裂原活化蛋白激酶核因子-κB 激活蛋白-1 狼疮性肾炎炎性反应

出版年：

2024

DOI：