Objective To investigate the inhibitory effect of curcumin on the inflammatory response in lupus nephritis mice by regulating the mitogen activated protein kinase(MAPK)/nuclear factor kappa B(NF-κB)/activator protein-1(AP-1)sig-naling pathway.Methods 16 LN mice were divided into LN group and experimental group,each 8 mice;Eight C57BL/6 mice were used as the control group.The experimental group mice were given 200 mg/kg curcumin by gavage,once a day;LN group and control group mice were given equal volume of physiological saline by gavage,once a day;Each group of mice was orally ad-ministered continuously for 4 weeks.Using a fully automated biochemical analyzer to measure the levels of Scr and BUN;Use the instructions of the BCA protein assay kit to measure urinary protein levels;Using ELISA method to measure the levels of IL-1β,IL-6,and TNF-α;Using Western Blot method to determine the expression of MAPK/NF-κB/AP-1 protein.Results The LN group and experimental group levels of Scr and BUN higher control group(P＜0.05);experimental group levels of Scr and BUN lower LN group(P＜0.05).The LN group and experimental group urinary protein levels higher control group(P＜0.05);experimental group urine protein level lower LN group(P＜0.05).The LN group and experimental group serum levels of IL-1β,IL-6,and TNF-α higher control group(P＜0.05);experimental group serum levels of IL-1β,IL-6,and TNF-α lower LN group(P＜0.05).The LN group and experimental group gray values of MAPK protein,NF-κB pro-tein,and AP-1 protein higher control group(P＜0.05);experimental group gray values of MAPK protein,NF-κB protein,and AP-1 protein lower LN group(P＜0.05).Conclusion Curcumin can inhibit the inflammatory response of LN mice and im-prove renal function by downregulating the expression of MAPK/NF-κB/AP-1 protein.
CurcuminMitogen activated protein kinaseNuclear factor-κBActivating protein-1Lupus nephritisin-flammatory reaction
维普
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