Exploring the Effect of Qinghua Decoction on the Scorching of Gastric Epithelial Cells in Rats with Chronic Atrophic Gastritis Based on NLRP3/Caspase-1/GSDMD Signaling Pathway
Objective To observe the effect of Qinghua Decoction(QHD)on the pyroptosis of gastric epithelial NOD-like re-ceptor protein 3(NLRP3)/cysteine protease-1(Caspase-1)/gasdermin D(GSDMD)in rats with chronic atrophic gastritis(CAG),and to explore the possible mechanism of QHY in the treatment of CAG.Method Rats were randomLy divided into a blank control group(n=8)and a modeling group(n=28).A combination of ammonia+sodium deoxycholate+ethanol ga-vage+high-fat and high-sugar diet+artificial climate chamber was used to establish a CAG rat model combined with dis-ease and TCM syndrome.After successful modeling,the models were randomLy divided into the model group,the vincristine treatment group,and the QHD treatment group(n=8).The gastric mucosa lesions were observed by HE staining.The expression of NLRP3,Caspase-1,β-catenin,and GSDMD mRNA were detected by Real-time PCR.The levels of serum 11.-1 β,IL-6,and IL-18 were detected by ELISA.Results Compared with the blank control group,the inflammatory cell infiltration and at-rophy of intrinsic glands in the gastric mucosa of the model rats were obvious;NLRP3,Caspase-1,and β-catenin mRNA ex-pression levels in the gastric mucosa tissues were significantly increased(P＜0.01);GSDMD mRNA expression levels were sig-nificantly decreased(P＜0.01);IL-1 β,IL-6 and IL-18 levels in serum were significantly increased(P＜0.01).Com-pared with the model group,the atrophy of intrinsic glands of gastric mucosa and the degree of inflammation in the QHD treat-ment group were significantly improved,and the expression levels of NLRP3,Caspase-1,and β-catenin mRNA were signifi-cantly decreased(P＜0.01).GSDMD mRNA expression level was increased(P＜0.01).The levels of serum IL-1 β,IL-6,and IL-18 decreased significantly(P＜0.01).Conclusion QHD can effectively improve the histopathological changes of gastric mucosa in CAG rats.Its mechanism may be related to the regulation of the NLRP3/Caspase-1/GSDMD signaling pathway,in-hibition of pyroptosis,and reduction of gastric mucosal inflammation level.
Qinghua Decoctionpyroptosischronic atrophic gastritisNOD-like receptor protein 3cysteine protease-1gasdermin D
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