Analyzing Mechanism of Huangqi(Astragali Radix)-Danshen(Salviae Miltiorrhizae Radix et Rhizoma)in Treatment of Pulmonary Fibrosis Based on Theory of"Lung Deficiency and Collateral Stasis"and Network Pharmacology
Objective To analyze the mechanism of action of Huangqi(Astragali Radix)-Danshen(Salviae Miltiorrhizae Radix et Rhizoma)in the treatment of pulmonary fibrosis based on the theoretical network pharmacology and molecular docking method of the theory of"lung deficiency and collateral stasis".Methods With the help of Traditional Chinese Medicine Systems Pharma-cology Database and Analysis Platform(TCMSP),it searched the active ingredients and the corresponding targets with the key-words of"Huangqi(Astragali Radix)"and"Danshen(Salviae Miltiorrhizae Radix et Rhizoma)"respectively,and converted the names of the targets of the drug ingredients into the corresponding gene names in the UniProt Protein Database.OMIM,Gene-Cards,TTD,DisGeNET and other disease gene databases were used to search and screen the target genes of pulmonary fibrosis diseases.Venny 2.1 online software was applied for mapping tool platform.The intersection of drug component targets and pul-monary fibrosis disease targets were taken to obtain drug-disease common target genes and draw the Wayne diagram.Cytoscape 3.10.0 mapping software was used to construct the traditional Chinese medicine-component-common target-disease gene net-work;and String database was used to construct the protein-protein-interaction(PPI)network,and the core targets were screened.At the same time,the core targets were screened out.The Metascape database was used to analyze the GO and KEGG enrichment of drug-disease intersecting targets,and the enrichment results were visualized through the microbiology platform,and molecular docking was verified with the help of AutoDock 1.5.7 software.Results The total number of active ingredients of Huangqi(Astragali Radix)-Danshen(Salviae Miltiorrhizae Radix et Rhizoma)was 85,and the core ingredients were quercetin,kaempferol,tanshinone Ⅵ and tanshinone ⅡA.The number of constituent targets was 1443,and the total number of target genes of drug-disease intersection was 85,and the core targets of disease were 12.The results of GO enrichment analysis yielded 1,370 items of bioprocesses,91 items of molecular functions and 60 items of cellular compositions.The results of KEGG enrichment analysis yielded 1,370 items of biological processes,60 items of CC,and the results of KEGG enrichment were visualized with the help of AutoDock 1.5.7 software.The results of GO enrichment analysis showed 1370 biological processes(BP),91 molecular functions(MF)and 60 cellular components(CC).A total of 169 signaling pathways were analyzed by KEGG enrichment analysis and the results of molecular docking validation showed that there was a relatively stable binding force between the small molecule ligands and protein receptors.Conclusion Huangqi(Astragali Radix)-Danshen(Salviae Miltiorrhizae Radix et Rhizoma)may exert its effects by regulating the protein expressions of MMP-9,TP-53,TNF,IL-1β,TGF-β1 through quercetin,kaempfer-ol,tanshinone ⅡA and other active ingredients,thus exerting its anti-lung fibrosis effect.
Huangqi(Astragali Radix)-Danshen(Salviae Miltiorrhizae Radix et Rhizoma)theory of"lung deficiency and collateral stasis"network pharmacologymolecular dockingpulmonary fibrosis
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