Objective:To observe the effect of different extraction methods on the improvement of sleep function in mice by Panax notoginseng.Methods:The effects of water extract,30％ethanol extract and 70％ethanol extract of Panax notoginseng on sleep in mice were evaluated by direct sleep test,prolonged pentobarbital sodium sleep time test,pentobarbital sodium subthreshold dose hypnosis test and barbital sodium sleep latency test.Results:Compared with the control group,each dose group of 70％ethanol extract of Panax notoginseng,the high dose group of 30％ethanol extract of Panax notoginseng and the high dose group of water extract of Panax notoginseng could prolong the sleep time of mice induced by pentobarbital sodium(P＜0.05 or P＜0.01),increase the number of mice falling asleep at the subliminal hypnotic dose of pentobarbital sodium(P＜0.05 or P＜0.001)and shorten the sleep latency of mice(P＜0.05 or P＜0.001).The sleep time,the number of sleeping mice and the sleep latency time were used as the main indicators to evaluate the effect of different extracts on improving sleep in mice.Compared with the control group,the low-dose group of 30％ethanol extract of Panax notoginseng and the low-dose group of water extract of Panax notoginseng had no significant effect on improving sleep in mice.Compared with the high-dose group of water extract of Panax notoginseng,the high-dose group of 70％ethanol extract of Panax notoginseng had significant difference in improving sleep in mice(P＜0.05).The contents of 5-hydroxytryptamine(5-HT)and γ-aminobutyric acid(GABA)in hypothalamus and serum of mice in the high dose of 70％ethanol extract group,the high dose of 30％ethanol extract group and the high dose of water extract group were significantly higher than those in control group(P＜0.05 or P＜0.01),and the content of dopamine(DA)was significantly lower than that in control group(P＜0.05 or P＜0.01).Conclusion:Panax notoginseng water extract,30％ethanol extract of Panax notoginseng and 70％ethanol extract of Panax notoginseng could improve the sleep of mice to a certain extent,among which 70％ethanol extract of Panax notoginseng has the best effect.
万方数据
维普
