Objective:To observe the effects of different concentrations of Radix Isatidis extract on cytokines IL-2,IL-4 and TNF-α in mycoplasma pneumoniae mice by establishing a mouse model of mycoplasma pneumoniae infection.Methods:Fifty SPF BALB/c healthy mice were randomly divided into control group,model group,high concentration of Radix Isatidis group(250mg/mL),medium concentration group(125mg/mL),low concentration group(62.5mg/mL),with 10 mice in each group.The mycoplasma pneumoniae infection model was established by nasal drip in the model group,and the mice in the control group were dripped with the same amount of PBS solution.The mice with successful modeling were treated with gavage for 7 days,and different concentration groups of Radix Isatidis were established to extract lung tissue.The pathological changes of lung tissue in mice were studied by hematoxylin-eosin(HE)staining.The expression levels of serum inflammatory factors IL-2,IL-4 and TNF-α were detected by flow microsphere array(CBD).Results:Compared with the control group,the lung wet weight index of the model group increased(P<0.05).Compared with the model group,the lung wet weight index and pathological score of the high,medium and low concentration groups of Radix Isatidis decreased(P<0.05).Compared with the control group,the concentration of IL-2,IL-4 and TNF-α in the model group increased(P<0.05).Compared with the model group,the concentration of IL-2 in the high concentration group,medium concentration group and low concentration group of Radix Isatidis decreased(P<0.05).Compared with the model group,the concentration of IL-4 in the low concentration group of Radix Isatidis decreased(P<0.05),and the concentration of TNF-α in the high concentration group of Radix Isatidis decreased(P<0.05).Conclusion:Mycoplasma pneumoniae infection in mice caused lung pathophysiological changes,cytokine IL-2,IL-4,TNF-α release,different concentrations of Radix Isatidis can inhibit cell release to varying degrees,play a protective role in lung tissue.
NETL
NSTL
万方数据
