Study on EST-SSR Genetic Characteristics of Medicinal Parts of Different Varieties of Rehmannia glutinosa
Objective The interspecific genetic characteristics of"Huaizhong No.1"a new variety of Rehmannia glutinosa and 4 mainstream varieties of medicinal parts in genuine producing area were studied by using the EST-SSR molecular marker method.Methods Optimizing the DNA extraction method to extract the DNA from the medicinal parts of R.glutinosa,the SSR-PCR primers of R.glutinosa were selected,Popgene32 software was used to calculate the genetic distance and genetic similarity coeffi-cient for analysis,and Ntsyspc2.1 was used to perform cluster analysis on the amplification results of R.glutinosa.Results The results showed that 5 pairs of primers with polymorphism were selected from 68 pairs of primers.A total of 97 bands were ampli-fied,including 78 polymorphic bands,the ratio of polymorphic bands was 80.41％.And the number of alleles detected by each pair of primers ranged from 10 to 21,with an average of 15.6.Effective alleles were 1.1697～1.3332,Nei's gene diversity was 0.0980～0.1980,Shannon index was 0.1461～0.3023,and the genetic distance of different breeds were ranged from 0.0665 to 0.1523.The cluster analysis results showed that when the coefficient of genetic similarity was 0.74,the 5 varieties were divided into 3 groups,Detoxification Beijing No.3 and Huaifeng were grouped together,Beijing No.3 and Jinjiu were grouped together,and Huaizhong No.1 was grouped separately.Conclusion The EST-SSR molecular marker method can be used to identify the genetic relationship between the medicinal parts of different varieties of R.glutinosa,and provide a scientific research basis for the study of genetic stability of R.glutinosa and variety identification.
NETL
NSTL
万方数据
维普
