Huangqi Guizhi Wuwu Decoction promotes the beige of white adipose by regulating the polarization of M1/M2 macrophages through α7nAchR
Objective To observe the effect of Huangqi Guizhi Wuwu Decoction on the beige of white adipose in obese mice.Methods 10 of 50 8-week-old male C57BL/6J mice were randomly selected as normal diet group(NF group),and the other 40 mice were induced by high-fat diet.40 obese mice successfully induced were randomly divided into high-fat diet group(HF group),β3 receptor agonist CL316,243 group(β3 group),CL316,243+Huangqi Guizhi Wuwu Decoction group(HGWD group)and CL316,243+Huangqi Guizhi Wuwu Decoction+α7nAchR selective antagonist α-BGT group(α-BGT group),given corresponding drugs respectively for 2 weeks.HE staining to observe the area of white adipose.PCR to detect the levels of thermogenic gene,M1 and M2 macrophage marker gene and α7nAchR gene in white adipose tissue.ELISA to detect the contents of white adipose tissue inflammatory factors.Results Compared to HF group,the weight of β3 group decreased,the area of adi-pocytes decreased and the thermogenic genes increased,which indicated that β3 receptor agonist promoted the beige of white adi-pose;however,the contents of marker genes of M1 and M2 macrophages and anti-inflammatory factors have not changed signifi-cantly,but the contents of pro-inflammatory factors have increased,indicating that the inflammatory state of obese body has not relieved;and the level of α7nAchR gene decreased.Compared to β3 group,HGWD group further increased the beige of white ad-ipose,significantly relieved the inflammatory state and increased the level of α7nAchR gene.However,the benefit effects of Huangqi Guizhi Wuwu decoction were weakened when co-administered with α-BGT.Conclusion Huangqi Guizhi Wuwu De-coction may inhibit the increase of M1 macrophages,promote the polarization of M2 macrophages,reduce the ratio of M1/M2,and then inhibit the inflammatory reaction,to improve the beige efficiency of white adipose in obese body.
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