Study pf Bushen Huoxue recipe on serum of VEGF/HIF-1α/TGF-β1/sfit-1 of posi-tive antiphospholipid antibodies Patients
Objective To study the effect of Bushen Huoxue Formula on peripheral blood VEGF,HIF-1α,TGF in APL positive RSA patients-β1.The expression levels of sflt-1 and miR-423-5p proteins are affected.Methods Sixty APL positive RSA patients were selected and administered medication before pregnancy.The observation group received the tonifying kidney and ac-tivating blood formula,while the control group received enteric coated aspirin,both receiving treatment for 3 months.Serum VEGF,HIF-1α,and TGF were measured before and after treatment in both groups-β1.Expression of sflt-1 and miR-423-5p proteins and integration of traditional Chinese medicine syndromes.Results The expression of VEGF protein was significantly increased in both groups after treatment;Compared with the control group,the expression of VEGF protein in the observation group was significantly higher than that in the control group;HIF-1 before and after treatment in both groups α There was no sig-nificant difference in protein expression levels;Two groups of TGF after treatment-β1.The protein expression was significantly increased,and compared with the control group,the observation group had TGF-β1.The protein expression significantly in-creased;After treatment,the expression of sflt-1 protein significantly decreased in both groups;Compared with the control group,the expression of sflt-1 protein in the observation group was significantly reduced.After treatment,the relative expression of miR-423-5p decreased in both groups,and the observation group was better than the control group(P<0.05).The obser-vation group compared the efficacy of traditional Chinese medicine syndromes,and the treatment group was better than the control group(P<0.05).Conclusion Bushen Huoxue Recipe can be used to regulate RSA patients before pregnancy by up regulating VEGF and TGF-β1 protein expression,downregulation of sflt-1,miR-423-5p protein expression,and other pathways to im-prove the adverse effects of APL on the endometrium;At the same time,it can effectively reduce the traditional Chinese medicine syndrome score and reduce the incidence of miscarriage after another pregnancy.
Kidney tonifying and blood activating formulaAnti phospholipid antibodiesRecurrent miscarriageSerum VEGFHIF-1αTGF-β1sflt-1miR-423-5p