生地大黄汤对脑出血大鼠的保护作用及对肠道菌群和短链脂肪酸的影响
The protective effect of Shengdi Dahuang Tang on rats with cerebral hemorrhage and its effects on gut microbiota and short chain fatty acids
杨文泰 1刘成海 1俞晓飞2
作者信息
- 1. 上海中医药大学附属曙光医院,上海 200120
- 2. 上海中医药大学附属龙华医院,上海 200032
- 折叠
摘要
目的 观察生地大黄汤对脑出血大鼠的保护作用及对肠道菌群和短链脂肪酸的影响.方法 30只大鼠随机分为假手术组、模型组、生地大黄汤组.于造模后6h生地大黄汤组灌胃生地大黄汤(18.51g·kg-1·d-1),假手术组和模型组灌胃等量生理盐水,每天1次,连续干预3d.分别于造模后3h、1 d、3d进行神经功能评分;造模后3d采集粪便标本,通过细菌16S rRNA高通量测序法比较各组肠道菌群的物种组成,气相色谱法检测大鼠粪便中短链脂肪酸(SCFAs)含量;造模后3d取脑组织测量脑含水量及TUNEL阳性细胞检测.结果 造模后3 d,与假手术组比较,模型组大鼠肠道菌群结构紊乱,短链脂肪酸(SCFAs)含量降低,神经功能缺损评分、脑含水量、脑组织细胞凋亡数升高;生地大黄汤可改善模型组大鼠的各项指标.结论 生地大黄汤对脑出血大鼠具有保护作用,该作用可能与恢复肠道微生物菌群正常构成和升高短链脂肪酸的浓度有关.
Abstract
Objective To observe the protective effect of Shengdi Dahuang Tang on rats with cerebral hemorrhage and its effects on gut microbiota and short chain fatty acids.Methods 30 rats were randomly divided into a sham surgery group,a model group,and a Shengdi Dahuang Tang group.After 6 hours of modeling,the Shengdi Dahuang Tang group was orally administered Shengdi Dahuang Tang(18.51g·kg-1·d-1),while the sham surgery group and model group were orally administered an equal a-mount of physiological saline,once a day,for a continuous intervention of 3 days.Perform neurological function scores at 3 hours,1 day,and 3 days after modeling;Three days after modeling,fecal samples were collected,and the species composition of gut microbiota in each group was compared using high-throughput 16S rRNA sequencing.The content of short chain fatty acids(SCFAs)in rat feces was detected by gas chromatography;Three days after modeling,brain tissue was taken to measure brain water content and TUNEL positive cells were detected.Results After 3 days of modeling,compared with the sham surgery group,the intestinal microbiota structure of the model group rats was disrupted,the content of short chain fatty acids(SCFAs)was re-duced,and the neurological deficit score,brain water content,and apoptosis number of brain tissue cells were increased;Shengdi Dahuang Tang can improve various indicators of the model group rats.Conclusion Shengdi Dahuang Tang has a protective effect on rats with cerebral hemorrhage,which may be related to restoring the normal composition of intestinal microbiota and increasing the concentration of short chain fatty acids.
关键词
生地大黄汤/脑出血/肠道菌群/短链脂肪酸/大鼠Key words
Shengdi Dahuang Tang/Cerebral hemorrhage/Intestinal microbiota/Short chain fatty acids/Rats引用本文复制引用
基金项目
国家自然科学基金青年基金(81503555)
上海市科委科研项目(19401972800)
上海市科委科技创新行动计划医学创新研究专项(21Y31920302)
上海市中医药高层次人才引领计划()
出版年
2024
NETL
NSTL
万方数据