高效液相色谱法同时测定还脑益聪方中5种川芎成分含量
Determination Content of 5 components in Huannao Yicong Decoction by HPLC
刘蔚然 1唐心恬 1何冠男 1梁晓东2
作者信息
- 1. 山东中医药大学,山东济南 250355
- 2. 山东中医药大学,山东济南 250355;中国中医科学院西苑医院老年病研究所,北京 100091
- 折叠
摘要
目的 建立还脑益聪方中阿魏酸、洋川芎内酯Ⅰ、洋川芎内酯A、藁苯内酯和欧当归内酯A 5种成分含量测定方法,探究其物质基础.方法 采用高效液相色谱法,色谱柱Agilent Extend-C18(4.6 mm ×250 mm,5pm);流动相:甲醇(A),0.3％磷酸溶液(B),乙腈(C);流速:1m L/min;柱温:30℃;检测波长:280nm.结果 还脑益聪方中川芎的5种化学成分均被检测到,建立的方法无阴性干扰,5种成分均达到良好的分离效果,标准曲线线性范围良好.结论 该方法简便稳定,重复性好,可用于还脑益聪方中川芎成分的定量检测,为还脑益聪方治疗AD的物质基础提供依据.
Abstract
Objective To establish a method for the determination of 5 components:Ferulic acid,Ligusticolide Ⅰ,Ligusticolide A,Ligustilide and Angelica lactone A in Huannao Yicong Decoction,and explore the material basis.Methods HPLC was per-formed on Agilent Extend-C18(4.6 mm ×250 mm,5μm)column.Mobile phase:methanol(A),0.3％phosphoric acid solu-tion(B),acetonitrile(C);Flow rate:1m L/min;Column temperature:30℃;Detection wavelength:280nm.Results A total of 5 chemical constituents of chuanxiong were detected in Huannao Yicong Decoction.The established method has no negative inter-ference,and the five components achieved good separation effect,and the linear range of standard curve is good.Conclusion This method is simple,stable and reproducible,and can be used for the quantitative detection of Chuanxiong in Huannao Yicong prescription,providing evidence for the material basis in the treatment of AD.
关键词
还脑益聪方/阿魏酸/洋川芎内酯Ⅰ/洋川芎内酯A/藁苯内酯/欧当归内酯A/含量测定Key words
Huannao Yicong Decoction/Ferulic acid/Ligusticolide Ⅰ/Ligusticolide A/Ligustilide and Angelica lac-tone A/Content determination引用本文复制引用
基金项目
山东省自然科学基金青年基金(ZR2016HQ34)
中国博士后科学基金面上项目(2015M571248)
北京市自然科学基金面上项目(7162169)
出版年
2024
国家科技期刊平台
NSTL
万方数据