Study on the myocardial protective effect of electroacupuncture pretreatment on isoprot-erenol induced acute myocardial ischemia in mice
Objective To explore the protective effect and possible mechanism of electroacupuncture pretreatment on isoproterenol(ISO)induced acute myocardial ischemia in mice.Methods 30 male Kunming mice were randomly divided into a control group,a model group,and an electroacupuncture pretreatment group,with 10 mice in each group.The model group and electroacupunc-ture pretreatment group induced acute myocardial ischemia model in mice by subcutaneous injection of ISO at multiple points on the back,while the control group received subcutaneous injection of physiological saline.The electroacupuncture pretreatment group treated mice with electroacupuncture in the bilateral"Later line 1 of forehead"area of the head point,with a density wave,frequency of 2 Hz/100 Hz,intensity of 2mA,and electroacupuncture for 30 minutes each time,once a day,for 7 consecutive days.After electroacupuncture on the 6th and 7th days,ISO models were injected.Two hours after the last administration of the drug for modeling,the mice were subjected to electrocardiogram testing;The myocardial tissue of mice was stained with hematoxy-lin eosin(HE)and masson to observe the morphological changes of myocardium,and the ultrastructural changes of myocardial cells were observed under transmission electron microscope;Enzyme linked immunosorbent assay(ELISA)was used to determine the levels of interleukin-6,tumor necrosis factor-α in mouse myocardial tissue,and creatin kinase-MB,cardiac troponin Ⅰ in serum.Results After modeling,the height of ECG T-wave and the displacement of J-point were significantly decreased in model group(P＜0.01),but the height of ECG T-wave and the voltage of J-point were not significantly decreased in electroa-cupuncture pretreatment group.Compared with the control group,the myocardial TNF-α,IL-6,serum CK-MB and cTnⅠ in model group were significantly increased(P＜0.01),and morphological observation showed that ischemic myocardial tissue was obviously damaged.Compared with the model group,the serum CK-MB,cTnⅠ,myocardial TNF-α and IL-6 levels in the electroacupuncture preconditioning group were significantly decreased(P＜0.01),and the degree of ischemic myocardial injury was significantly reduced by morphological observation.Conclusion Electroacupuncture pretreatment of"Later line 1 of forehead"can obviously improve the deviation of ECG T wave and J point in mice with acute myocardial ischemia,reduce the degree of myo-cardial pathological injury,and reduce the contents of CK-MB,cTnⅠ,IL-6 and TNF-α in myocardium,thus playing a pro-tective role.
Myocardial ischemiaElectroacupuncture PretreatmentIsoproterenolLater line 1 of forehead
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