Sequencing of transcriptome in roots,stems and leaves of Dendrobium nobile and analysis of key enzymes genes in lignin synthesis pathway
Objective To study the differentially expressed genes in the roots,stems,and leaves of Dendrobium nobile,analyze the expression characteristics of key enzyme genes in the lignan synthesis pathway,and explore the molecular mechanism of lignan synthesis in Dendrobium nobile.Methods Total RNAs were extracted from the roots,stems and leaves of Dendrobium nobile,and transcriptome sequencing and bioinformatics analysis were performed using DNBSEQ platform.Results There were the more differ-entially expressed genes in root-vs-leaf of Dendrobium nobile,while there were the less differentially expressed genes in leaf-vs-stem.There were 879 genes shared by three sample comparison groups.In GO classification,the differentially expressed genes were mainly classified into biological processes,while in KEGG enrichment analysis,the differentially expressed genes were mainly enriched into α-Linolenic acid metabolic pathway,glycerophospholipid metabolic pathway,cyano amino acid metabolic pathway,etc.Among the lignan synthesis related genes,there were two CAD genes,one DIR gene and four UGT genes whose ex-pression trends in roots,stems and leaves were consistent with the lignan content trend.Conclusion This study obtained differen-tially expressed gene in the roots,stems and leaves of Dendrobium nobile,especially the genes related to lignan synthesis were screened,and a network diagram of lignan synthesis in Dendrobium nobile was drawn,laying the foundation for conducting re-search on the molecular mechanism of lignan synthesis in Dendrobium nobile.
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