Effect of Huangqin Decoction regulating TLR2/NF-κB/NLRP3 pathway on pyroptosis of ulcerative colitis
Objective To investigate the regulatory effect of Huangqin Decoction on the pyroptosis pathway of TLR2/NF-κB/NLRP3/in ulcerative colitis.Methods HCT-116 cells were randomly divided into blank group,model group,low,medium and high herbal serum group(15％,20％and 25％herbal serum)LPS+ATP was used to construct the pyroptosis model,Cell growth was detected by cck-8 method,cell apoptosis was detected by flow cytometry,IL-18,IL-1β and TNF-α levels were detec-ted by Elisa,mRNA expression of IL-1β,NLRP3,Caspase-1,IL-18,ASC and GSDMD were detected by RT-PCR.The protein levels of TLR2,p-NF-κB-p65and GSDMD were detected by WB.Results Compared with the model group,the ser-um cell vitality of the three groups was obviously increased(P＜0.05),and the apoptosis rate was significantly decreased(P＜0.01).Compared with model group,serum levels of IL-18,IL-1β and TNF-α in three groups were obviously decreased(P＜0.05).Compared with model group,mRNA expressions of Caspase-1,NLRP3,ASC and IL-18 in medium-dose group were obviously decreased(P＜0.05),while mRNA expressions of IL-1β,NLRP3,Caspase-1,IL-18,ASC and GSDMD in high-dose group were significantly decreased(P＜0.01).Compared with model group,the protein expressions of TLR2,P-NF-Kb-p65 and GSDMD in medium and high dose groups were significantly decreased(P＜0.01).Conclusion The mecha-nism of Huangqin Decoction inhibiting pyrodeath in ulcerative colitis may be the regulation of TLR2/NF-κB/NLRP3 pathway.
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