Antagonistic Effect and Mechanism of Raw Zizyphi Spinosae Semenon on Anxiety In-duced by Conditional Fear in Rats
Objective To investigate the antagonistic effect and mechanism of raw Zizgphi Spinosae semen on anxiety induced by conditioned fear in rats.Methods SD rats were randomly divided into 4 groups:control group,model group,diazepam group(3.6 mg/kg)and sour jujube seed group(5.0 g/kg).The anxiety model was duplicated by the conditional fear method.After replication of the model,the drug was administered by gavage for 10 days.One hour after the last administration,the anxiety-like behavior of the rats with anxiety caused by conditional fear was evaluated by open-field test(OFT)and elevated cross maze test(EPM);The content of cortisol(CORT)in serum,CORT,brain-derived neurotrophic factor(BDNF),5-hydroxytryptamine(5-HT),norepinephrine(NE),dopamine(DA),glutamate(GLU)and γ-Aminobutyric acid(GABA)content and calculate the ratio of Glu/GABA.Results In the behavioral experiment,compared with the control group,the rats in the model group had fewer times of crossing the grid,moving time in the central grid and entering the central grid in the OFT ex-periment(P＜0.05 or P＜0.01);In the EPM experiment,the percentage of open/total time and the percentage of open/total times decreased(P＜0.01).Compared with the model group,the number of cross-grid and central grid movement time of rats in diazepam group increased(P＜0.01);The total arm entry times,the percentage of open/total time and the percentage of open/total times in EPM of rats in diazepam group and raw Zizyphi Spinosae group increased(P＜0.05 or P＜0.01).In the Elisa method experiment,compared with the control group,CORT,Glu content and Glu/GABA content in the cortex,hippocam-pus and amygdala of the model group increased,GABA content decreased(P＜0.05 or P＜0.01).Compared with the model group,the contents of CORT,NE,Glu and GABA in the cortex,hippocampus and amygdala of rats in diazepam group and raw Zizyphi Spinosae group decreased,the ratio of Glu/GABA increased(P＜0.05 or P＜0.01).In diazepam group,the content of DA in amygdala decreased(P＜0.05),but the content of 5-HT in hippocampus decreased(P＞0.05);The content of 5-HT in the hippocampus of the raw Zizyphi Spinosae group decreased(P＜0.05),but the content of DA in the amygdala decreased(P＞0.05).There was no significant difference in the content of CORT in serum between the experimental animals in each group(P＞0.05).Conclusion Raw Zizyphi Spinosae can effectively improve the anxiety-like behavior of conditioned anxiety rats,and its anti-anxiety mechanism may be related to the changes of CORT,BDNF and neurotransmitter content in cortex,hippo-campus and amygdala.
Raw Zizyphi Spinosae SemenAnxietyConditioned fearEffect
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