The Effect of QingJieHuaGong Decoction(QJHGD)on Colon Macrophage Polarization and Glycolysis Key Kinases in Rats with Severe Acute Pancreatitis
Objective To explore the repair Effect and possible mechanism of QJHGD on colon injury in severe acute pancreatitis(SAP)from the Perspective of Glucose Metabolism of Intestinal Macrophages.Methods The SAP rat model was replicated by in-jecting rain frog extract and lipopolysaccharide into the pancreatic capsule.The pathological changes of the pancreatic tissue in the blank group,model group,and traditional Chinese medicine group were observed using HE staining;Enzyme linked immunosor-bent assay for detecting serum amylase,IL-6,IL-10,TNF-α Expression level;The M1/M2 polarization subtype of macro-phages in the lamina propria of SAP colon mucosa was detected by immunofluorescence;Western blot was used to detect the ex-pression levels of key kinase HK2,PKM2,PDK1,and LDHA proteins in colon glycolysis;PCR was used to detect the expression levels of PKM2,HK2,PDK1,and LDHA mRNA in the colon.Results Pancreatic pathology and ELISA experiments indicate that Qingjie Huachuang Formula can alleviate pancreatic edema,reduce the release of inflammatory factors,and inhibit pancreatic and colonic inflammatory reactions in SAP rats.The immunofluorescence results showed that compared with the blank group,the pro-portion of M1 macrophages in the lamina propria of the model group was significantly increased,and the proportion of M2 macro-phages had no significant change.Compared with the model group,the compound Chinese medicine could effectively reduce the proportion of M1 macrophages and increase the proportion of M2 macrophages(P＜0.05).The WB results showed that compared with the blank group,the expression levels of HK2,PKM2,PDK1,and LDHA proteins in the model group were significantly in-creased.Compared with the model group,the expression levels of these proteins in the treatment group were significantly de-creased(P＜0.05);The PCR results showed that compared with the blank group,the mRNA expression levels of HK2,PKM2,PDK1,and LDHA in the model group were significantly increased.Compared with the model group,the mRNA expression levels of HK2,PKM2,and PDK1 in the colon tissue of rats in the treatment group were significantly decreased(P＜0.05).Conclusion QJHGD can effectively inhibit colonic inflammatory response in rats with severe acute pancreatitis,possibly by regulating the ex-pression of key glycolytic kinases such as PKM2 and inducing colonic macrophage M2 polarization to exert anti-inflammatory effects.
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