基于"温阳达表"探讨麻黄附子细辛汤对SSc模型小鼠皮肤及肺组织纤维化的作用机制
The mechanism of Mahuang FuZi XiXin Decoction on skin and lung tissue fibrosis in SSc model mice based on the"Wen Yang Da Biao"
周于捷 1李想 1王振亮1
作者信息
- 1. 河南中医药大学中医学院,河南郑州 450046
- 折叠
摘要
目的 观察麻黄附子细辛汤(MFXT)对SSc模型小鼠皮肤及肺组织中HyP含量,皮肤组织中MMP-1及TIMP-1表达的影响,探讨"温阳达表"法治疗系统性硬化症(systemic sclerosis,SSc)组织纤维化的作用机制.方法 将50只雌性BALB/c小鼠随机分为正常组,模型组,麻黄附子细辛汤高、中、低剂量组,每组10只.除正常组外,各组均通过皮下注射博莱霉素(bleomycin,BLM)注射液制备SSc小鼠模型.成模后,麻黄附子细辛汤高、中、低剂量组分别给予麻黄附子细辛汤混悬液9.9,3.3,1.1g/kg,均按0.2ml/10g灌胃容量给药,连续21d.取各组小鼠皮肤及肺组织进行病理形态学观察,ELISA法检测皮肤及肺组织中HyP含量,WB法测定皮肤组织中MMP-1和TIMP-1的表达.结果 麻黄附子细辛汤能明显改善SSc小鼠的组织病理变化,显著影响SSc小鼠皮肤及肺组织中HyP含量(P＜0.05),调节MMP-1及TIMP-1的表达(P＜0.05).结论 麻黄附子细辛汤可下调HyP含量以及调控MMP-1和TIMP-1的平衡,改善SSc模型小鼠皮肤及肺组织纤维化程度,有效治疗SSc组织纤维化,为"温阳达表"法指导治疗SSc组织纤维化提供实验依据.
Abstract
Objective To observe the effects of Mahuang FuZi XiXin Decoction(MFXT)on the content of HyP,MMP-1 and TIMP-1 expression in skin and lung tissues of SSc model mice,and to explore the mechanism of action of"Wen Yang Da Biao"method in the treatment of SSc tissue fibrosis.Methods Fifty female BALB/c mice were randomly divided into normal group,model group,Mahuang FuZi XiXin decoction high,medium and low dose group(n=10).Except for the normal group,SSc mouse models were prepared by subcutaneous injection of bleomycin(BLM)injection.After molding,Mahuang FuZi XiXin de-coction high,medium and low dose groups were given Mahuang FuZi XiXin decoction suspension 9.9g/kg,3.3g/kg,1.1g/kg,all according to 0.2ml/10g gavage capacity for 21 days.The skin and lung tissues of each group of mice were taken for pathologi-cal morphological observation,the HyP content in skin and lung tissues was detected by ELISA method,and the expression of MMP-1 and TIMP-1 in skin tissues was measured by WB method.Results Mahuang FuZi XiXin decoction could significantly improve the histopathological changes of SSc mice,significantly affect the HyP content(P＜0.05)in the skin and lung tissues of SSc mice,and regulate the expression of MMP-1 and TIMP-1(P＜0.05).Conclusion Mahuang FuZi XiXin decoction can downregulate the content of Hyp,regulate the balance of MMP-1 and TIMP-1,improve the degree of skin and lung tissue fibro-sis of SSc model mice,and effectively treat SSc tissue fibrosis,which provides an experimental basis for the"Wen Yang Da Biao"method for the treatment of SSc tissue fibrosis.
关键词
温阳达表/麻黄附子细辛汤/组织纤维化Key words
Wen Yang Da Biao/MaHuang FuZi XiXin Decoction/Tissue fibrosis引用本文复制引用
基金项目
国家自然科学基金(81072716)
河南省郑州市协同创新专项(2023XTCX049)
出版年
2024
NETL
NSTL
万方数据