Pharmacokinetics and tissue distribution of five active components from Yiqi Huoxue Gushen Granules in rats
Objective To investigate the pharmacokinetics and tissue distribution of palmatine,jatrorrhizine,berberine,epiber-berine and formononetin of Yiqi Huoxue Gushen Granules(YHGG)in rats.To provide evidence for further clarifing the pharma-codynamic material basis of this prescription.Methods The HPLC-MS/MS method was established to detect the drug concentra-tion in rat plasma and tissues after oral administration of 4.75 g/kg YHGG.A Kromasil C18 column(3.5 μm,2.1 mm × 100 mm)as the stationary phase and the mobile phase consisted of acetonitrile-0.1％formic acid in water at the flow rate of 0.3 mL/min,the injection volume was 5 μL.The electrospray ionization source(ESI)was set in positive and negative ion mode re-spectively by multiple reaction monitoring(MRM)scanning.The main pharmacokinetic parameters were calculated by WinNonlin 3.3 software.Results The method met the detection requirements of biological samples.After intragastric administration of com-pound YHGG,the five components absorbed into the blood rapidly and the plasma concentrations can reached the peak within 30 min.Furthermore,all components owned short half-life and quick elimination rate in vivo.The Cmax and AUC0-t of each component were 4.87～233.12 μg/L,16.11～493.13 h/(μg·L),respectively.The results of tissue distribution showed that 5 components distributed rapidly,widely but unevenly in vivo.They were more distributed in the stomach and small intestine,fol-lowed by liver,kidney and lung,and less in the spleen and heart.Conclusion The method was stable and reliable which will provide reference for the clinical application of YHGG.
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