Objective In order to improve the germination rate and utilization rate of Isatis indigotica Fort.seeds,the quality difference of Isatis indigotica Fort.seeds with different colors was analyzed and a seed coating agent suitable for Isatis indigotica Fort.seeds was screened.Methods Observation and determination of the external form of 6 Isatis indigotica Fort.producing seeds and clarity,according to the color classification investigation on sale,Yuncheng and folk music origin seeds to take off water con-tent,thousand seed weight,germination potential,germination rate,germination index and other indicators,and uses the 5 kinds of seed germination experiment indicated that composite coating,the indexes of germination and biomass.The quality standard of color grading of Isatis indigotica Fort.seed was determined,and a seed coating agent suitable for Isatis indigotica Fort.seed coat-ing was screened.Results Isatis indigotica Fort.seeds were divided into three colors:yellow,black and yellow,and purplish-black.The quality indexes were purplish-black group,black and yellow group,and yellow group in turn.The seed germination percentage and root biomass,such as root length,root diameter,plant height and root to shoot ratio,were higher in T2 concentra-tion of D seed coating agent than in control Conclusion Isatis indigotica Fort.seeds can be divided into three colors:yellow,black and yellow and purple and black,and purple and black Isatis indigotica Fort.seeds are the best.D coating agent T2 gradi-ent treatment of Isatis indigotica Fort.seeds had the best effect.