目的 研究加味过敏煎调控Ⅰ型超敏反应大鼠药效及作用机制，以明确加味过敏煎治疗Ⅰ型超敏反应免疫分子机制及作用靶点。方法 通过卵白蛋白(OVA)建立Ⅰ型超敏反应大鼠模型。将造模成功的动物分为模型组、地塞米松阳性对照组(8。4×10-5g/kg)、加味过敏煎高、中、低剂量组(25。44 g/kg、12。72 g/kg、6。36 g/kg)。另设不造模的空白组。末次给药后，测定大鼠血清中免疫球蛋白E(IgE)、组胺、白介素-4(interleukin-4，IL-4)、肿瘤坏死因子(tumor necrosis factor-α，TNF-α)的因子含量;RT-PCR 法测定大鼠脾脏中 IFN-γ、T 细胞中 T-box(T-box expressed in T cells，T-bet)、IL-4、GATA 结合蛋白 3(GATA family of transcription factors3，GATA3)、白细胞介素-17(interleukin-17，IL-17)、白细胞介素-10(interleukin-10，IL-10)、叉头状转录因子 P3(forkhead box protein-3，FOXP3)的 mRNA 表达水平。结果 与模型组比较，加味过敏煎高、中剂量组明显降低Ⅰ型超敏反应大鼠血清中IgE(P＜0。01，P＜0。05)、组胺含量(P＜0。05)，升高IFN-γ水平(P＜0。05)，并且高剂量组能有效降低血清中IL-4水平(P＜0。05);RT-PCR结果显示，与模型组比较，加味过敏煎高、中、低剂量组可以上调Ⅰ型超敏反应大鼠脾组织T-bet(P＜0。05，P＜0。01)、1L-10(P＜0。05)、FOXP3(P＜0。05)mRNA表达，高、中剂量组显著促进IFN-γ mRNA表达(P＜0。05)。同时加味过敏煎高、中、低剂量组下调IL-17mRNA表达(P＜0。05)，高剂量组显著抑制IL-4、GATA3 mRNA表达(P＜0。05)。结论 加味过敏煎可以通过调节Th1/Th2细胞、Th17/Treg细胞平衡抑制炎症反应，进而影响免疫功能，达到抑制Ⅰ型超敏反应的作用。
Study on pharmacodynamics and mechanism of Jiawei Guomin Decoction on type Ⅰ hy-persensitivity
Objective Study the efficacy and mechanism of Jiawei Guomin decoction in regulating type Ⅰ hypersensitivity Immune molecular mechanism and target of hypersensitivity.Methods To establish the mode of type Ⅰ hypersensitivity by cvalban in(OVA)Animals that will be successfully modeled that divided into model group,dexamethasone positive control group(8.4 × 10-5 g/kg),high,medium and low dose group(25.44 g/kg,12.72 g/kg,6.36 g/kg)of Jiawei Guomin Decoction.Set up a blank group without modeling.After the last administration,then measured IgE,histamine,IL-4,IFN-γ of rat serum.The mRNA expression of IFN-γ,T-bet,IL-4,GATA3,IL-17,IL-10,Foxp3 in the spleen of was determined by the RT-PCR.Results Compared with the model group,the serum IgE(P＜0.01,P＜0.05)and histamine contents(P＜0.05)of type Ⅰhypersensitivity rats were significantly reduced in the high and medium dose groups of Jiawei Guomin Decoction,and the IFN-γ level was increased(P＜0.05).Moreover,the IL-4 level in the serum was effectively reduced in the high dose group(P＜0.05).RT-PCR results showed that compared with the model group,Jiawei Guomin Decoction high,medium and low dose groups could up-regulate the expression of T-bet(P＜0.05,P＜0.01),IL-10(P＜0.05)and FOXP3(P＜0.05)mRNA in spleen tissue of type Ⅰ hypersensitivity rats,and high and medium dose groups significantly promoted the expression of IFN-γ mRNA(P＜0.05).At the same time Jiawei Guominjian high,medium and low dose group down-regulated IL-17 mRNA ex-pression(P＜0.05),high dose group significantly inhibited IL-4,GATA3 mRNA expression(P＜0.05).Conclusion Jiawei Guomin Decoction can inhibit inflammatory response by regulating the balance of Th1/Th2 cells and Th17/Treg cells,thereby affecting immune function and inhibiting type Ⅰ hypersensitivity.
Jiawei Guomin decoctionType Ⅰ immediate hypersensitivityPharmacodynamic studyMechanism study
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