Experimental study on the regulation of TGF-β1,SMAD2 and BMP2 expression in bili-ary duct cells by DHLX
Objective To explore the effects of DHLX on the expression levels of TGF-β1,SMAD2 and BMP2 in intrahepatic bile duct cells,and to analyze the mechanism of its action in relieving cellular inflammation and preventing cholelithiasis.Meth-ods The drug serum of DHLX was prepared,and the inflammation state model of intrahepatic bile duct cells was induced by li-popolysaccharide(LPS),and the drug serum of DHLX was used to intervene.The experiment was divided into control group,model group and DHLX group.The expression level of LL-18 inflammatory factor was detected by ELISA after 72 h.Western blot was used to detect the expression of TGF-β1,SMAD2 and BMP2 proteins in bile duct cells,and RT-PCR was used to de-tect the mRNA expression levels of TGF-β1,SMAD2 and BMP2 in bile duct cells.Results Compared with the control group,the content of IL-18 inflammatory factor and the expression levels of TGF-β1,SMAD2 and BMP2 protein and mRNA in the model group were significantly increased,with statistical significance(P＜0.01 or P＜0.05).Compared with the model group,the expression level of IL-18 inflammatory factor and the protein and mRNA expressions of TGF-β1,SMAD2 and BMP2 in DHLX group were significantly decreased,and the differences were statistically significant(P＜0.01 or P＜0.05).Conclusion DHLX can significantly reduce the expression levels of TGF-β1,SMAD2 and BMP2,delay the inflammatory reaction process of bile duct cells,prevent fibrosis,and thus play a role in the prevention and treatment of cholelithiasis.
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