天山假狼毒化学成分及抗菌活性研究
Study on Chemical Constituents and Antibacterial Activity of Stelleropsis tianschanica(Pobed)
张梦雪 1方容 2杨金花 2雷亮 2徐玲 3宫立孟 4宋红萍5
作者信息
- 1. 湖北中医药大学药学院,湖北武汉 430061
- 2. 武汉市第四医院,湖北武汉 430033
- 3. 湖北省药品监督检验研究院,国家药品监督管理局中药质量控制重点实验室,湖北武汉 430075
- 4. 武汉药品医疗器械检验所,湖北武汉 430075
- 5. 湖北中医药大学药学院,湖北武汉 430061;武汉市第四医院,湖北武汉 430033
- 折叠
摘要
目的 研究瑞香科植物天山假狼毒Stelleropsis tianschanica(Pobed)的化学成分及抗菌活性.方法 采用柱色谱的分离方法对天山假狼毒的95％乙醇提取物进行分离纯化,利用核磁共振NMR、高分辨质谱等方法,结合理化鉴定化合物结构.采用滤纸片法对不同浓度的单体化合物的抗菌活性进行筛选.结果 从天山假狼毒中分离鉴定11个化合物,分别为:(E)-11-methoxy-11-oxoundec-2-enoic acid(1)、Methyl-(E)-9-oxooctadec-10-enoate(2)、3-Hydroxyphthalimide(3)、Dimethyl hexadecanedioate(4)、Octadecane-1,18-dimethylester(5)、壬二酸氢甲酯(6)、正十六烷酸(7)、bothrioclinin(8)、Daphnolon(9)、β-谷甾醇(10)、芝麻素(11).化合物7(128 μg/ml)对金黄色葡萄球菌有一定抑制活性,抑菌圈大小为8.65 mm.化合物9(128 μg/ml和64 μg/ml)对鲍曼不动杆菌有抑制活性,抑菌圈大小分别为12.65 mm和8.05 mm.结论 化合物1～9为首次从天山假狼毒中分离得到;其中化合物1～3的碳谱数据为首次报道.化合物7和化合物9均表现出一定的抗菌活性.
Abstract
Objective To study the chemical constituents of Stelleropsis tianschanica(Pobed),a Thymelaeaceae plant.Methods Using column chromatography to separate and purify the pure methanol extract of Stelleropsis tianschanica(Pobed)and the struc-ture of the compound was rationalized and identified using nuclear magnetic resonance NMR and high-resolution mass spectrome-try.The filter paper method was used to screen the antibacterial activity of monomeric compounds at different concentrations.Re-sults Eight compounds were obtained and identified as(E)-11-methoxy-11-oxoundec-2-enoic acid(1)、Methyl-(E)-9-oxooctadec-10-enoate(2)、3-Hydroxyphthalimide(3)、Dimethyl hexadecanedioate(4)、Octadecane-1,18-dimethyl-ester(5)、9-methoxy-9-oxononanoic acid(6)、n-Hcxadecane acid(7)、Bothrioclinin(8)、Daphnolon(9)、β-sitostcrol(10)、Sesamin(11).Compound 7(128 μg/ml)had some inhibitory activity against Staphylococcus aureus,with a size of 8.65mm of the antibacterial ring,and compound 9(128 μg/ml and 64 µg/ml)had inhibitory activity against Acinetobacter bau-mannii,with antibacterial ring of 12.65 mm and 8.05 mm.Conclusion Compounds 1～9 were obtained from this plant for the first time and the 13 C-NMR data of compounds 1～3 were reported for the first time.Compounds 7 and 9 showed certain antimicrobial activity.
关键词
天山假狼毒/化学成分/结构鉴定/抗菌活性Key words
Stelleropsis tianschanica Pobed/Chemical constituents/Structural identification/Antimicrobial activity引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据