Study on the effect of Jiawei Xiaoyao San on Neural Cell Protrusion through hepatocellu-lar carcinoma exosomes
Objective Exploring whether Jiawei Xiaoyao San(JWXYS)has a regulatory effects on the growth of neural cell pro-trusions through exosomes.Methods The non-contact co culture system of neural cells and astrocyte in vitro was used to simu-late the interleaving and influencing relationship between cells in the central nervous system in vivo,then added the exosomes sep-arately derived from hepatoma cells and hepatoma cells dealt with JWXYS containing serum.Finally,compare the effects of the two on the length and branch of nerve cell dendrites,and the number of excitatory synapses and sunaptic associated proteines,etc in the neurocyte Astrocyte co culture system.Results Compared with the blank group,the length and branch of the nerve cell dendrites,as well as the number of excitatory synapses and sunaptic associated proteines intervened by exosomes derived from hepatoma cells were decreased.However,the exosomes derived from hepatoma cells which were dealt with JWXYS containing ser-um can significantly increase the length and branch of the nerve cell dendrites,and the number of excitatory synapses and sunaptic associated proteines in the co-culture system.Conclusion JWXYS could repair and protect the abnormal damage of neural cell protrusions caused by the exosomes derived from hepatoma cells.In other words,JWXYS may intervene and repair damaged nerve cells through the exosomes pathway.
Jiawei Xiaoyao SanExosomes derived from hepatoma cellsNeural cell protrusion
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