Effect of Neohesperidin on NLRP3/NF-κB Signaling Pathway on mice with non-alco-holic steatohepatitis
Objective To explore the therapeutic effect and mechanism of Neohesperidin(NHP)on non-alcoholic steatohepati-tis(NASH)mice.Methods 24 SPF C57BL6 male mice were randomly divided into normal group,model group and NHP group(40mg/kg).High-fat and high-cholesterol diet(HFHC)were fed to bulid the NAFLD model,and each treatment group was given corresponding drugs at the same time.After 12 weeks,conducted glucose tolerance test and insulin tolerance test,calculat-ed mice body weight,liver weight and liver index,collected serum and liver tissue samples to detecte in total cholesterol(TC),triglyceride(TG),alanine aminotransferase(ALT),aspartic acid aminotransferase(AST).Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR)and Western blot were used to observe the gene and protein expressions of inflam-matory body 3(NLRP3)and protein expression of phosphorylated nuclear transcription factor-κB(NF-κB)in liver.Hematox-ylin-eosin(HE)staining and Oil red staining to observe the pathological morphological changes of liver.Results Compared with normal group,the contents of TC,TG,ALT and AST in serum,the mRNA and protein expression of NLRP3 and NF-κB in liver and the protein expression of P-NK-κB in model group were significantly increased(P＜0.05).Compared with model group,the contents of TC,TG,ALT and AST in serum,the mRNA and protein expression of NLRP3 and NF-κB in liver and the protein expression of P-NF-κB in NHP group were distinctly decreased(P＜0.05).HE staining and Oil red staining indi-cated that NHP could significantly reduce the degree of liver steatosis.Conclusion NHP may treat NASH by inhibiting NLRP3/NF-κB signaling pathway and alleviating inflammatory response.
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