首页|基于仿生手段和气质联用技术研究炮制方法对五味子滋味和气味的影响

基于仿生手段和气质联用技术研究炮制方法对五味子滋味和气味的影响

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目的 利用两种仿生手段联合气相色谱-质谱联用(GC-MS)联用技术,表征不同炮制方法下五味子滋味和气味的变化。方法 制备醋蒸、酒蒸、盐炒和蜜炒的4种五味子炮制品。采用电子舌测定五味子样品的滋味,利用电子鼻和顶空气相质谱仪器分析五味子样品的气味。采用主成分分析法对数据进行分析。结果 表明电子舌能够检测到五味子的酸、涩、苦、咸及甜味,其中酸味为主。经炮制后甜味消失、酸味有明显降低,酒蒸制后涩味明显增加,盐制后咸味变化较大。电子鼻传感器响应结果说明样品中含有芳香类成分、含硫化合物、氮氧化物及短链烷烃,其中醋五味子的气味与其他炮制品的差异较大。GC-MS共鉴定出32种化合物,包括烷烃类、醇类、醚类和萜烯类,其中萜烯类含量较高,有衣兰烯、花柏烯、雪松烯、香柑油烯等。挥发性成分的总相对含量经蒸制处理后均有降低,尤其是醋制品,但倍半萜类成分有所升高。经盐炒后炮制品中单萜含量升高,而蜜炒后总相对含量及分布基本不变。结论 五味子经过蒸、炒等炮制方法处理后,滋味和气味都会受到影响。醋、酒、盐和蜂蜜等辅料的添加对滋味的影响较大。该研究通过探索五味子在炮制过程中的气味和滋味的变化,客观阐释五味子五味俱全的科学内涵,为进一步联系五味子五味和物质基础之间的关系奠定了基础。
The effect of processing methods on the taste and smell of Schisandra chinensis based on bionic method and GC-MS
Objective In this paper,two biomimetic methods combined with Gas Chromatography-Mass Spectrometry(GC-MS)were used to characterize the changes in flavor and odor of Schisandra chinensis under different processing technology.Meth-ods The flavor of vinegar-steamed,wine-steamed,salt-fried,and honey-fried Schisandra chinensis samples prepared were determined by the electronic tongue,and the odor of the four kinds of processed conducts is determined by the electronic nose and the GC-MS instrument.This study adopted principal component analysis to analyze the data.Results The experimental results showed that the electronic tongue was able to detect the sour,astringent,bitter,salty,and sweet flavors of Schisandra chinensis,with the sour taste predominating.The sweetness disappeared and the acidity decreased significantly after processing,while the astringency increased notably after wine steaming processing,and the saltiness changed significantly after salt processing.The re-sponses of the electronic nose sensor indicated that the samples contained aromatic components,sulfur-containing compounds,nitrogen oxides,and short-chain alkanes,while the odor of the vinegar-steamed Schisandra chinensis differed significantly from that of the other processed conducts.A total of 32 compounds were identified by GC-MS,including alkanes,alcohols,ethers,and terpenes,of which the content of terpenes was high,including ylangene,cupressene,cedrene,and citrus oilene.The total relative content of volatile components decreased after steaming processing,especially in the vinegar-steamed products,but the content of sesquiterpenes increased.The content of monoterpene increased after processed products fried with salt,while the total relative content and its distribution remained basically unchanged after honey frying processing.Conclusion The flavor and odor of Schisandra chinensis are affected after steaming,frying,and other processing.The addition of auxiliary ingredients such as vin-egar,wine,salt,and honey exerts a significant impact on the flavor.This study provides an objective interpretation of the scien-tific connotation of the five-flavored Schisandra chinensis,and lays a solid foundation for the further relationship between the five flavors and the material basis of Schisandra chinensis.

Schisandra chinensisProcessing methodsElectronic noseElectronic tongueGC-MS

王依、张灏潆、赵子平、杨善、张楠淇、李婉莹、宋明杰、王英平

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吉林农业大学中药材学院人参新品种选育与开发国家地方联合工程研究中心,吉林长春 130118

五味子 炮制品 电子舌 电子鼻 气相色谱-质谱法(GC-MS)

2024

时珍国医国药
时珍国医国药杂志社

时珍国医国药

北大核心
影响因子:0.887
ISSN:1008-0805
年,卷(期):2024.35(3)