Study on the role of Platycodon grandiflorum in the treatment of Sjogren's syndrome
Objective To study whether Platycodon grandiflorum can form a synergistic and attenuated effect on Zengye Decoction in the treatment of Sjogren's syndrome,and to explore the corresponding mechanism.Methods NOD mice with spontaneous Sjogren's syndrome were randomly divided into model group,Zengye Decoction group and Zengjie group,and ICR mice were se-lected as control group.The control group and the model group were given normal saline by gavage,and the other groups were giv-en corresponding drug treatment.Drug treatment for 7 weeks.The salivary flow rate of the mice was detected during the gavage.After the gavage,the mice were sacrificed and the submandibular glands were removed for pathological evaluation.The expression of AQP5 and VIP in the submandibular glands was determined by immunohistochemistry.The proportion of CD8+T cells in cervi-cal lymph nodes was detected by flow cytometry.The changes of intestinal microecology in mice were detected by third-genera-tion sequencing.Results Compared with the control group,the salivary flow rate of mice in the model group decreased signifi-cantly at 16 weeks of age(P=0.0027),the expression of VIP and AQP5 in submandibular gland decreased(P＜0.0001,P=0.0013),the pathological score of submandibular gland and the proportion of CD8+T cells in cervical lymph nodes increased,and the intestinal microecology suggested that the intestinal microecology a diversity index of the model group was lower than that of the control group.Compared with the model group,the expression of VIP and AQP5 and the salivary flow rate of mice in the Zengyc Decoction group and the Zengjie group were increased,the pathological score of submandibular gland and the proportion of CD8+T cells in cervical lymph nodes were decreased.Zengye Decoction had no significant effect on the abundance of intestinal microecology,but Zengjie group could significantly improve the intestinal microecology a diversity Shannon and Pielou index;compared with the Zengye decoction group,the salivary flow rate and the expression of VIP and AQP5 in the Zengjie group were further increased.Conclusion Platycodon grandiflorum can enhance the effect of Zengye Decoction on the treatment of Sjogren's syndrome,promote the upward distribution of body fluid,and improve the intestinal microecology,which has the effect of"carry-ing body fluid upward".
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