Study on the protective effect and mechanism of Fuyuan Xingnao Decoction on blood-brain barrier in MCAO model rats
Objective To investigate the effects of Fuyuan Xingnao Decoction on the blood-brain barrier in acute ischemic cere-bral infarction model rats and its mechanism.Methods 40 SD rats were randomly divided into the sham operation group,model group,Fuyuan Xingnao Decoction group,and Nimodipine group.Middle cerebral artery embolization was established in the other groups to prepare the ischemic cerebral infarction model except for the sham operation group.The sham operation group and model group were given 0.9％sodium chloride solution,Fuyuan Xingnao decoction group and Nimodipine group were given the corre-sponding equivalent dose of drugs,and gavage twice a day,continuous gavage for 3 days.Bederson neural function score was used.The pathological changes in brain tissue were observed by hematoxylin-eosin staining.The expressions of ZO-1 and Claudin-5 were observed by immunohistochemistry.The protein expression of MMP-2,MMP-9,ZO-1,and Claudin-5 in rat brains was detected by Western blot.The expression levels of MMP-2,MMP-9,ZO-1 and Claudin-5 genes in rat brain were detected by RT-qPCR.Results Compared with sham operation group,the neurological impairment of rats in model group was higher.Compared with sham operation group,the protein and mRNA expressions of MMP-2 and MMP-9 in brain tissue of model group were increased(P＜0.05,P＜0.01),while the protein mRNA expressions of ZO-1 and Claudin-5 were de-creased(P＜0.05,P＜0.01).Compared with model group,the mRNA expressions of MMP-2 and MMP-9 protein in ische-mic penumbra of brain tissue in Fuyuan Xingnao Decotion group were significantly decreased(P＜0.05,P＜0.01).The mRNA expressions of ZO-1 and Claudin-5 protein were significantly increased(P＜0.05,P＜0.01).Conclusion Fuyuan Xingnao Decoction can protect the permeability of the blood-brain barrier,and may inhibit the expression of MMP-2 and MMP-9,and inhibit the degradation and loss of ZO-1 and claudin-5 in tight junction protein,so as to protect the structure and function of the blood-brain barrier.
Fuyuan Xingnao DecoctionCerebral infarctionBlood-brain barrierMetal matrix proteinaseTight junc-tion protein
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