Study on the Effect and Mechanism of Shugan Hewei Formula on Esophageal Motility in GERD Rats
Objective Study on the effect of Shugan Hewei formula on esophageal motility function in GERD rats.Methods Rats were randomly divided into blank group,model group,Shugan Hewei formula group,and rabeprazole group.The animal model was established using semi pyloric ligation and cardiac myotomy.HE staining was used to observe esophageal tissue,and RT-qPCR,Western blot,and immunohistochemistry were used to detect the mRNA and protein expression of PAR-2,IP3R,RyR,MLCK,CaM.In vitro muscle strip tissue experiments were conducted to investigate the effect of PAR-2 agonists on muscle strips.Results Compared with the model group,the expression of PAR-2 mRNA and protein was reduced(P＜0.05),while the expression of CaM and MLCK mRNA and protein was increased(P＜0.05),and the expression of IP3R protein was in-creased(P＜0.05)in the Shugan Hewei formula group and the rabeprazole group.At the same time,the expression of IP3R mR-NA was increased(P＜0.05)in the Shugan Hewei group;The results of in vitro muscle strip tissue experiments showed that the change rate of muscle strip tension in the Shugan Hewei formula group and rabeprazole group increased(P＜0.05),while the effect of PAR-2 agonist on the two groups of muscle strips decreased(P＜0.05).Conclusion The mechanism of Shugan Hewei Formula in treating GERD may be related to the regulation of PAR-2 signaling pathway.
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