Study on the compatibility mechanism of Buyang Huanwu Decoction on improving myo-cardial injury in diabetic
Objective To observe the protective effect of Buyang Huanwu Decoction and its compatibility on myocardial injury in diabetic mice and its possible mechanism,so as to provide theoretical basis and experimental basis for clinical application.Meth-ods Diabetic model mice were prepared with high-fat diet combined with streptozotocin,and the levels of blood glucose,TC,TG,LDH,CK-MB,IL-2,TNF-α,MDA and SOD were detected;HE and Masson staining were used to detect the patholog-ical changes of myocardial tissue,Western Blot method was used to detect the protein expression levels of MFN2,DRP1 and MFF in myocardial tissue;To observe the effect of Buyang Huanwu Decoction and its different compatible prescriptions on diabetic myocardial injury,and preliminarily analyze its mechanism.Results Compared with the model group,the blood lipids in the Buy-ang Huanwu Decoction group were significantly lower(P＜0.05),and the serum LDH and CK-MB were significantly lower(P＜0.01).The results of pathological staining showed that compared with the normal group,the gaps between the myocardial cells in the model group became larger and the arrangement was uneven,there was myocardial damage,and the myocardial collagen fi-bers increased.The results of Western blot showed that,compared with the model group,administration of each group up-regu-lated the expression of MFN2 protein,and down-regulated the expression of DRP1 and MFF protein.Conclusion Buyang Hua-nwu Decoction and its compatibility can reduce myocardial injury in diabetic mice,and its mechanism may be related to inhibiting inflammatory reactin,alleviating oxidatire stress injury,and Regnlating the expression of mitochondrial dynamic proteins.
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