The combined analysis of proteome and transcriptome of the Scolopendra subspinipes mu-tilans based on sexual differences
Objective Through transcriptomic and proteomic association analysis of individual males and females of the Scoropen-dra subspinipes mutilans L.Koch,this article explored the differences between male and female individuals,providing a basis for possible differences in pharmacological effects and genetic improvement.Methods 12 S.mutilans(6 males and 6 females)were used as the experimental material,we conducted transcriptomic sequencing based on the high-throughput sequencing platform Il-lumina NovaSeq and quantitative analysis of the proteome using Data Independent Acquisition(DIA)technology.Differential ex-pression genes(DEGs)and differential abundance proteins(DAPs)were subjected to correlation analysis.Results A total of 3602 significantly DEGs were identified between male and female individuals of the S.mutilans.Among these,2898 genes were down-regulated and 704 genes up-regulated were in males.Analysis of differential protein abundance between male and female individuals revealed 354 DAPs,with 81 up-regulated and 273 down-regulated proteins in males.In separate and combined an-alyses of the transcriptome and proteome of the S.mutilans,it was found that male and female individuals exhibited significant differences in genes related to toxin activity,metabolism,cholesterol metabolism,fatty acid metabolism,and synthesis.Conclu-sion The differential expression between male and female individuals of the S.mutilans mainly focuses on pathways related to en-ergy storage,nutrient metabolism,and toxin activity,and these differences may impact the growth rates and medicinal quality of male and female individuals.
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