Effect of Electroacupuncture on Ferroptosis in Cortical Nerve Cells of Rats with Post Stroke Spasticity
Objective To observe the effects of electroacupuncture on the ultrastructure,ferrous ion(Fe2+),malondialdehyde(MDA),and glutathione(GSH)expression in cortical neuronal cells of rats with post stroke spasticity(PSS),exploring the mechanisms of ferroptosis in electroacupuncture for post stroke spasticity.Methods A rat model of cerebral PSS was produced u-sing the modified ZeaLonga thread bolus+internal capsule injection of NMDA method.Thirty healthy adult male SD rats were randomly divided into sham surgery group(SS group),model group(M group)and electroacupuncture group(EA group).The electroacupuncture group took points on the affected side"Yanglingquan and Quchi",1 time/day,30min/time,for 7d.Zea-lon-ga Neurological Function Score and Modified Ashworth Dystonia Score were recorded,mitochondrial changes were observed by transmission electron microscopy,cortical iron ion aggregation was observed by Prussian blue staining,and cortical Fe2+and MDA contents were detected by biochemical kits,and cortical GSH contents were detected by ELISA in rats.Results Zea-longa Neurological Function Score:compared with the model group,the score of the electroacupuncture group was reduced(P＜0.05);Modified Ashworth Dystonia Score:compared with the model group,the score of the electroacupuncture group was reduced(P＜0.05);Transmission electron microscopy:compared with the model group,the damage to the cortical neuronal cells of the elec-troacupuncture group was reduced,the degree of mitochondrial atrophy was reduced,and mitochondrial cristae were more obvi-ous.Prussian blue staining:compared with the model group,cortical iron aggregation was significantly reduced in the electroacu-puncture group.Laboratory test results:compared with the model group,Fe2+and MDA content decreased in the electroacupunc-ture group(P＜0.01),and GSH content increased(P＜0.01).Conclusion Electroacupuncture can improve post stroke spas-ticity,and its mechanism of action may be related to electroacupuncture inhibiting ferroptosis of cortical nerve cells,exerting cere-bral protective effects and promoting neurological recovery.
ElectroacupuncturePost stroke spasticityCortical nerve cellsPrussian blue stainingFe2+MDAGSHFerroptosis
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