Objective To analyze the molecular mechanism of Jiedu Huayu Granules(JDHY)in improving liver inflammation in rats with liver failure by regulating ROS-NLRP3 signaling pathway.Methods Thirty-six male Wistar rats were randomly divid-ed into control group,model group and JDHY group,with 12 rats in each group.A rat model of acute liver failure was established by intraperitoneal injection of D-galactosamine(D-GalN)combined with lipopolysaccharide(LPS).JDHY group was given JDHY(57.55 g·kg-1·d-1)before modeling,continuous intragastric administration for 72 h and continued to 24 h after model-ing.Rats in each group were sacrificed 24 h after modeling.Serum and tissue samples were collected.Serum ALT,AST and TBIL levels were measured by automatic biochemical analyzer.Serum IL-1β and IL-18 were measured by ELISA.ROS pro-duction was evaluated.The expression of NLRP3 and GSDMD in liver tissue was analyzed by immunohistochemistry.The expres-sion of NLRP3,GSDMD,GSDMD-N,ASC and Caspase-1 in liver tissue was analyzed by Western blotting.Results JDHY could effectively improve liver function(ALT,AST,TBIL)(P＜0.05),reduce the excessive production of ROS(P＜0.05)and the release of inflammatory factors(IL-1β,IL-18)in rats with liver failure.In addition,compared with the model group,JDHY reduced the expression of NLRP3,GSDMD,GSDMD-N,ASC and Caspase-1 in liver tissue of rats with liver failure(P＜0.05).Conclusion JDHY can inhibit the excessive activation of ROS-NLRP3 signaling pathway,reduce the release of pro-inflammatory factors,alleviate the level of liver inflammatory stress and antagonize liver failure.
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