Study on the embryonic developmental toxicity of aqueous extracts of Taxilli Herba based on the influence of host trees
Objective To study the embryonic toxicity of the aqueous extracts of Taxilli Herba from Morus alba,Salix babylonica,Camellia oleifera,Nerium indicum,Melia azedarach and Toxicodendron trichocarpum to zebrafish embryos,hoping to provide a theoretical basis for the safe use of Taxilli Herba.Methods The normally developing zebrafish embryos at 6 hours post-fertilisati-on(hpf)was used as an animal model.Zebrafish embryos were treated with gradient concentrations of the aqueous extracts of Taxilli Herba from Morus alba,Salix babylonica,Camellia oleifer,Nerium indicum,Melia azedarach and Toxicodendron tricho-carpum,and zebrafish embryo mortality was counted at 72 hpf.GraphPad Prism 8.0 was used to plot the dose-toxicity curve after calculating the median lethal concentration(LC50)and the 10％lethal concentration(LC10).Three concentration groups of low-,medium-,and high-dose were established according to their 1/2LC10,LC0,LC10,and treated to zebrafish embryos at 6 hpf.Then,the number of voluntary movements within 1 minute was then counted at 24 hpf.Hatching rate,the eye size,and body length were counted and measured at 72 hpf.Results The results show that the LC50 values of aqueous extracts of Taxilli Herba from six hosts:M.alba,S.babylonica,C.oleifera,N.indicum,M.azedarach,and T.trichocarpum were 6.28,3.86,2.14,1.02,1.00 and0.36 mg/mL,respectively.The LC10 values were4.77,2.76,1.16,0.67,0.62 and0.25 mg/mL,respective-ly.In comparison to the blank group,no significant alterations were observed in the number of voluntary movements,hatching rate,eye size,and body growth length in all the dose groups of Taxilli Herba extracted from M.alba(P＞0.05).In contrast,the medium-and high-dose groups of the aqueous extracts of Taxilli Herba from the other five hosts showed significant changes(P＜0.05,P＜0.01).Conclusion Taken together,with the exception of Taxilli Herba from M.alba,Taxilli Herba from the oth-er five hosts exhibited varying degrees of embryonic toxicity to zebrafish embryos.
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