首页|益肺宣肺降浊方调控小胶质细胞极化改善血管性痴呆神经炎症的机制研究

益肺宣肺降浊方调控小胶质细胞极化改善血管性痴呆神经炎症的机制研究

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目的 观察益肺宣肺降浊方对血管性痴呆大鼠海马组织小胶质细胞极化以及调控TLR4/NF-κB信号通路的影响.方法 75只老鼠随机分为假手术组(SHAM组)、模型组(MOD组)、益肺宣肺降浊方低剂量组(LDY组),益肺宣肺降浊方中剂量组(MDY组),益肺宣肺降浊方高剂量组(HDY组),吡拉西坦组(PIR组).运用2-VO法(双侧颈总动脉永久性结扎法)造模,造模后经药物干预后行水迷宫检验大鼠学习记忆行为;HE染色、透视电镜分析海马病理形态学;TUNEL法检验海马CA1区细胞凋亡,免疫荧光双标染色检测小胶质细胞极化表型蛋白CD16/32+/Iba-1+、CD206+/Iba-1+共标情况,RT-PCR检测小胶质细胞M1/M2分泌促/抗炎因子TNF-α、IL-1β以及IL-4、TGF-B的基因表达,western blot分析小胶质细胞极化iNOS和Arginase-1以及TLR4/NFKB信号通路相关蛋白表达.结果 与模型组对比,益肺宣肺降浊方组对海马组织神经细胞凋亡以及神经元损害的比值降低(P＜0.05),其中以高剂量效果最佳(P＜0.01),小胶质细胞Ml型CD16/32阳性细胞数下降(P＜0.05),上述可被益肺宣肺降浊汤逆转.结论 益肺宣肺降浊汤神经保护作用通过抑制炎症相关TLR4/NF-κB信号通路,促进小胶质细胞向M2型转化保护神经元改善血管性痴呆.
Study on the mechanism of VD neuroinflammation by regulating microglial polarization
Objective The effect of Yifei Xuanfei Jiangzhuo formula on microglial polarization and regulation of TLR4/NF-KBsignaling pathway in hippocampal tissues of rats with vascular dementia was observed.Methods 75 mice were randomlydivid-ed into sham surgery group(SHAM group),model group(MOD group),low-dose group(LDY group),medium-dose group(MDY group),high-dose group(HDY group),and piracetam group(PIR group).The 2-VO method(bilateral common ca-rotid artery permanent ligation)was used to test the learning and memory behavior of rats by water maze before and after model-ing.HE staining and fluoroelectron microscopy analysis of hippocampal pathological morphology;TUNEL method to detect apopto-sis in hippocampal CA1 region,immunofluorescence double-standard staining to detect microglia polarized phenotypic proteins CD16/32+/Iba-1+and CD206+/Iba-1+co-standardization,RT-PCR to detect gene expression of microglia M1/M2 secre-tion of proactive/anti-inflammatory factors TNF-α,IL-1β,IL-4 and TGF-B,western blot to analyze microglia polarized iNOS and Arginase-1 and TLR4/NFKB signaling pathway-related protein expression.Results Compared with the model group,the ratio of prohibitive lung xuan lung turbidity reduction formula to apoptosis and neuronal damage in hippocampal tissue was reduced(P＜0.05),among which the best effect was at high doses(P＜0.01),and the number of microglia M1 CD16/32 positive cells decreased(P＜0.05),which could be reversed by yifei xuanfei jianzhuo decoction.Conclusion The neuroprotec-tive effect of Yilungxuan lung turbidity decoction protects neurons by inhibiting inflammation-related TLR4/NFKB signaling path-way,promoting the transformation of microglia to M2 type,and improving vascular dementia.

yifei xuanfei jianzhuoVasculardementiaMicrogliaNeuroinflammation

朱健敏、陈炜、匡龙娇、卓桂峰、吴林

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广西中医药大学研究生院,广西南宁 530001

广西中医药大学第一附属医院,广西南宁 530023

江西中医药大学研究生院,江西南昌 330004

广西中医药大学科学实验中心,广西南宁 530001

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益肺宣肺降浊方 血管性痴呆 小胶质细胞 神经炎症

国家自然科学基金地区基金国家自然科学基金地区基金广西中医药大学研究生教育创新计划项目广西中医脑病临床研究中心广西中医药重点学科建设项目广西中医基础研究重点实验室课题

8216088582060844YCBXJ2022023桂科AD20238028GZXK-Z-20-1317-259-49-01

2024

10.3969/j.issn.1008-0805.2024.05.24

时珍国医国药
时珍国医国药杂志社

时珍国医国药

北大核心
影响因子：0.887
ISSN：1008-0805
年,卷(期)：2024.35(5)