The effect of Qingrehuashitiaoshu decoction granules based on VIP signal on preventing colonic mucosal barriers injury in DSS-induced colitis in mice
Objective Based on the changes of vasoactive intestinal peptide(VIP)signal and its receptors,to observe the pro-tective effect and mechanism of Qingrehuashitiaoshu decoction(QRHSTSD)on colonic mucosal barriers injury in DSS-induced colitis mice model.Methods Twenty-four C57BL/6 male mice were selected.Six mice in the normal group were given pure wa-ter for free drinking.The remaining 18 mice were divided into model group,5-ASA group and QRHSTSD group.The mice were given 2.5％dextran sodium sulfate(DSS)aqueous solution for free drinking,and were given the same amount of pure water or 5-ASA suspension(0.52g/kg/d),QRHSTSD granules(1.69g/kg/d)for 7 days.The body weight,disease activity index(DAI)scores,colonic length and HE staining pathological section of the distal colon tissue of mice in each group were observed.Serum levels of IL-2 and IL-4 were detected by ELISA.The level of IL-10 mRNA in colon tissue was detected by q-PCR.The levels of sIgA,Claudin2,MUC2,VIP,VPAC1 and VPAC2 were detected by Western Blot.Results Compared with the nor-mal group,the body weight of the mice in the model group decreased,the DAI score increased,and the colonic length was short-ened.There was no significant difference in serum IL-2 and IL-4.The levels of IL-10 mRNA,sIgA,Claudin2,MUC2,VIP and VPAC2 in colon tissue were decreased,and the level of VPAC1 in colon tissue was increased.Compared with the model group,QRHSTSD and 5-ASA group could reduce the DAI scores and restore the colonic length of mice,and the difference was statistically significant.In addition,QRHSTSD could restore the body weight of mice more than 5-ASA.The mice in the QRH-STSD and 5-ASA group had tighter colonic mucosal epithelial structure and less inflammation.There was no significant differ-ence in serum IL-2,and only QRHSTSD could increase serum IL-4 level in mice.The levels of IL-10 mRNA,SIgA,Clau-din2,MUC2,VIP and VPAC2 in colon tissue were restored and the level of VPAC1 was decreased in QRHSTSD group and 5-ASA group.Conclusion QRHSTSD has a preventive effect on DSS-induced colitis to some degree,and can restore the damaged colonic mucosal mechanical,chemical,and immune barriers,and can regulate immunity.The regulation of mechanical barrier may be driven by VIP-VPAC2.The regulation of chemical barrier is related to VPAC2.And the regulation of immune barrier is related to VPAC1.
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