Effects of SHCY peptide on endothelial dysfunction in SHR rats through PI3K/Akt/eNOS pathway
Objective To investigate the protective effect of traditional Chinese medicine compound SHCY peptide on the vascular endothelium of hypertensive rats through the PI3K/Akt/eNOS pathway.Methods Thirty-five 15-week-old male SHR rats were randomly divided into 5 groups:model group,perindopril group,and low,medium,and high dose groups of the SHCY pep-tide,each group of seven.Seven Wistar rats of the same age were selected as the normal group.The non-invasive blood pressure detection system measured the artery systolic and diastolic blood pressure of the tail arteries of rats in each group before and after intragastric administration.Echocardiography was performed to examine the changes of heart structure in each group.HE staining was used to observe the pathological changes of the thoracic aorta in each group;Enzyme linked immunosorbent assay(ELISA)was used to detect levels of angiotensin Ⅱ(Ang Ⅱ),endothelin-1(ET-1),nitric oxide(NO);Western Blot was used to de-tect the protein expression of phosphatidylinositol 3-kinase(PI3K),phosphorylated phosphatidylinositol 3-kinase(p-PI3K),protein kinase B(Akt),phosphorylated protein kinase(p-Akt),endothelial nitric oxide synthase(eNOS)and phosphorylated endothelial nitric oxide synthase(p-eNOS)protein expression.Results After 8 weeks of administration,the systolic blood pres-sure of rats in the low,medium,and high dose groups of the SHCY peptide and the perindopril group were lower than those in the model group(P＜0.05).The echocardiographic results showed that compared with the model group,the end-systolic left ven-tricular diameter(LVIDs),end-diastolic left ventricular diameter(LVIDd),end-diastolic left ventricular anterior wall thick-ness(LVAWd)and end-diastolic left ventricular posterior wall thickness(LVPWd)of SHCY peptide group and perindopril group were significantly reduced(P＜0.05).The HE results showed that compared with the model group,the swelling endotheli-al cells in the rats treated with the SHCY peptide group and perindopril group was improved,and the arrangement of vascular en-dothelial cells tended to be smooth.ELISA results showed that compared with the model group,the serum NO content in the per-indopril group,the middle and high dose groups of the SHCY peptide were significantly increased(P＜0.05),while Ang Ⅱ,ET-1 contents in the serum were significantly reduced(P＜0.05).Western Blot results showed a significantly increase in protein expression levels of p-PI3K/PI3K,p-Akt/Akt,and p-eNOS/eNOS.Conclusion The traditional Chinese medicine com-pound SHCY peptide may promote the release of NO by affecting the PI3K/Akt/eNOS signaling pathway,reduce the levels of Ang Ⅱ,ET-1,improve endothelial function injury in SHR,and have a significant intervention effect on blood pressure in SHR.
SHREndothelial injuryPI3K/Akt/eNOS signaling pathwayChinese medicine compound extract
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