Optimization of extraction process and fingerprint analysis of detoxifying and revitalizing agents
Objective To optimize the water extraction process of detoxification and rejuvenating mixture,and establish quality standards and fingerprints to identify its chemical composition.Methods The orthogonal test method and L9(34)experimental de-sign were used to investigate the effects of water addition,extraction times,extraction time and other factors on the extraction process,and the water extraction process of the detoxification and renormalization mixture was optimized.The content determina-tion method was established by HPLC method,and the fingerprint was established,and the total content of baicalin,hesperidin,ointment yield and total flavonoids were used as comprehensive evaluation indexes.Results The best water extraction process was determined to be 10 times of water,decoction for 3 times,each time for 1.5h,ultraviolet spectrophotometry results showed that the average content of total flavonoids of detoxification and rejuvenating mixture was 33.423 μg/ml,and HPLC results showed that the average total content of baicalin and hesperidin was 771.971 μg/ml.Conclusion This study provides a basis for the determi-nation of the water extraction process of detoxification and rejuvenating mixture,and the established quality standard can also stab-ly control its active substance composition,which lays a foundation for subsequent research.
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