Study on Ligustri Lucidi Fructus before and after steaming with wine improves diabetic renal fibrosis based on TGF-β1/Smads pathway
Objective To study the effect and mechanism of Ligustri Lucidi Fructus before after steaming with wine on renal fibro-sis in rats with diabetic nephropathy based on transforming growth factor-β1(TGF-β1)/Smads pathway.Methods Eight male SD rats were randomly selected as control group,and the rest rats were fed with high-fat diet and intraperitoneal injection of 1％streptozotocin(65 mg/kg)to establish a diabetic nephropathy(DN)model.DN model rats were randomly divided into model group,metformin group,low and high dose of Ligustri Lucidi Fructus group,low and high dose of wine Ligustri Lucidi Fructus group,with 8 rats in each group.The corresponding drugs were given to the rats in each administration group,rats in control group and model group were given the same volume of distilled water,once a day for 8 weeks.Fasting blood glucose was detected by blood glucose meter,24 h urine protein was detected by coomassie brilliant blue method,serum creatinine(Scr)and blood u-rea nitrogen(BUN)levels were determined by ELISA.Masson staining was used to observe the pathological situation of renal tis-sue.qRT-PCR was used to determine the mRNA expression of TGF-β1,Smad 3,and Smad 7 in renal tissue.Western blotting was used to determine the expression of TGF-β1,Smad 3,phosphorylated Smad 3(p-Smad 3),Smad 7 and phosphorylated Smad 7(p-Smad 7)in renal tissue.Results Compared with the control group,the blood glucose,24 h urine protein,Scr and BUN levels in the model group were significantly increased(P＜0.01).Renal tissue showed collagen fiber proliferation and mod-erate fibrosis around the glomeruli,severe mesangial matrix proliferation,basement membrane thickening,and glomerular atro-phy.TGF-β1 and Smad 3 mRNA levels and the protein expressions of TGF-β1,Smad 3 and p-Smad 3 in renal tissue were significantly increased(P＜0.01),Smad 7 mRNA level and the protein expressions of Smad 7 and p-Smad 7 in renal tissue were significantly reduced(P＜0.01).Compared with model group,the renal fibrosis and renal injury of rats were improved to varying degrees in Ligustri Lucidi Fructus group and wine Ligustri Lucidi Fructus group.The levels of blood glucose,24 h urine protein,SCr,BUN were significantly reduced(P＜0.05 or P＜0.01),TGF-β1 and Smad 3 mRNA levels and the protein ex-pression of TGF-β1,Smad 3 and p-Smad 3 in renal tissue were significantly reduced(P＜0.05 orP＜0.01).Smad 7 mRNA level and the protein expression of Smad 7 and p-Smad 7 in renal tissue were significantly increased(P＜0.05 orP＜0.01),the improvement effect of wine Ligustri Lucidi Fructus was better than that of Ligustri Lucidi Fructus.Conclusion Ligustri Lucidi Fructus after steaming with wine may enhance the therapeutic effect on renal fibrosis in DN rats by regulating TGF-β1/Smads pathway.
Ligustri Lucidi FructusSteaming with wineDiabetic nephropathyRenal fibrosisTGF-β1/Smads pathway
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